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Literature summary extracted from

  • Bachmann, B.O.; Townsend, C.A.
    Kinetic mechanism of the beta-Lactam synthetase of Streptomyces clavuligerus (2000), Biochemistry, 39, 11187-11193.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.3.4 deoxyamidinoproclavaminate product inhibition Streptomyces clavuligerus
6.3.3.4 diphosphate product inhibition Streptomyces clavuligerus
6.3.3.4 N2-(carboxymethyl)-L-arginine competitive to N2-(carboxyethyl)-L-arginine Streptomyces clavuligerus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.3.4 0.153
-
 ATP pH 7.5 Streptomyces clavuligerus
6.3.3.4 0.22
-
 N2-(carboxyethyl)-L-arginine pH 7.5 Streptomyces clavuligerus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.3.4 N2-(carboxyethyl)-L-arginine + ATP Streptomyces clavuligerus early step in clavulanic acid biosynthesis AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.3.4 Streptomyces clavuligerus P0DJQ7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.3.4 recombinant protein expressed in E. coli Streptomyces clavuligerus

Reaction

EC Number Reaction Comment Organism Reaction ID
6.3.3.4 ATP + L-N2-(2-carboxyethyl)arginine = AMP + diphosphate + deoxyamidinoproclavaminate it has been proposed that L-N2-(2-carboxyethyl)arginine is first converted into an acyl-AMP by reaction with ATP and loss of diphosphate, and that the beta-lactam ring is then formed by the intramolecular attack of the beta-nitrogen on the activated carboxy group, in a sequential ordered bi-ter kinetic mechanism Streptomyces clavuligerus
a

Storage Stability

EC Number Storage Stability Organism
6.3.3.4 -20°C, 50 mM Tris-HCl, 10% glycerol, 2 mM dithiothreitol, 0.01 mM EDTA, pH 7.5, stable Streptomyces clavuligerus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.3.4 N2-(carboxyethyl)-L-arginine + ATP mechanism Streptomyces clavuligerus AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?
6.3.3.4 N2-(carboxyethyl)-L-arginine + ATP early step in clavulanic acid biosynthesis Streptomyces clavuligerus AMP + diphosphate + deoxyamidinoproclavaminate
-
 ?