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Literature summary extracted from
- Identification of coenzyme M biosynthetic phosphosulfolactate synthase: a new family of sulfonate-biosynthesizing enzymes (2002), J. Biol. Chem., 277, 13421-13429.
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 4.4.1.19 | K137N | no measurable enzymatic activity | Methanocaldococcus jannaschii |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.19 | EDTA | - |
Methanocaldococcus jannaschii |  |
| 4.4.1.19 | LiCl | weak | Methanocaldococcus jannaschii | |
| 4.4.1.19 | MgCl2 | inhibition above 10 mM | Methanocaldococcus jannaschii | |
| 4.4.1.19 | additional information | further weak inhibitors, not inhibitory: K+, Na+, NH4+ | Methanocaldococcus jannaschii | |
| 4.4.1.19 | phosphoenolpyruvate | substrate inhibition above 5 mM | Methanocaldococcus jannaschii | |
| 4.4.1.19 | sulfite | substrate inhibition above 3 mM | Methanocaldococcus jannaschii | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.19 | 9.8 | - |
phosphoenolpyruvate | - |
Methanocaldococcus jannaschii | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 4.4.1.19 | Mg2+ | required | Methanocaldococcus jannaschii | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.19 | 28000 | - |
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence | Methanocaldococcus jannaschii |
| 4.4.1.19 | 32000 | - |
3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence | Methanocaldococcus jannaschii |
| 4.4.1.19 | 86700 | - |
gel filtration | Methanocaldococcus jannaschii |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.19 | additional information | Methanocaldococcus jannaschii | catalyzes the first step in coenzyme M biosynthesis | ? | - |
? | |
| 4.4.1.19 | sulfite + phosphoenolpyruvate | Methanocaldococcus jannaschii | - |
(2R)-O-phospho-3-sulfolactate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 4.4.1.19 | Methanocaldococcus jannaschii | Q57703 | - |
- |
Reaction
| EC Number | Reaction | Comment | Organism | Reaction ID |
|---|---|---|---|---|
| 4.4.1.19 | (2R)-2-O-phospho-3-sulfolactate = phosphoenolpyruvate + sulfite | catalyses the Michael addition of sulfite to phosphoenolpyruvate, requires Mg2+, specifically requires phosphoenolpyruvate and its broad alkaline pH optimum suggests that it uses sulfite rather than bisulfite | Methanocaldococcus jannaschii |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 4.4.1.19 | 5 mM MgCl2 restores activity of EDTA-treated enzyme | Methanocaldococcus jannaschii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.19 | 2.4 | - |
recombinant enzyme | Methanocaldococcus jannaschii |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 4.4.1.19 | 4°C, 20 mM Tris-HCl, 1 mM MgCl2, pH 8.0, 2 months, no loss of activity | Methanocaldococcus jannaschii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 4.4.1.19 | additional information | catalyzes the first step in coenzyme M biosynthesis | Methanocaldococcus jannaschii | ? | - |
? | |
| 4.4.1.19 | sulfite + phosphoenolpyruvate | - |
Methanocaldococcus jannaschii | (2R)-O-phospho-3-sulfolactate | - |
? | |
| 4.4.1.19 | sulfite + phosphoenolpyruvate | - |
Methanocaldococcus jannaschii | (2R)-O-phospho-3-sulfolactate | enantiomeric ratio of 84:16 R-isomer:S-isomer | ir | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 4.4.1.19 | trimer | 3 * 32000, SDS-PAGE, 3 * 28000, deduced from gene sequence | Methanocaldococcus jannaschii |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.19 | 8.5 | - |
- |
Methanocaldococcus jannaschii |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 4.4.1.19 | 6 | 11 | - |
Methanocaldococcus jannaschii |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 4.4.1.19 | 1.9 | - |
phosphoenolpyruvate | - |
Methanocaldococcus jannaschii | |
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