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Literature summary extracted from

  • Tonello, F.; Ascenzi, P.; Montecucco, C.
    The metalloproteolytic activity of the anthrax lethal factor is substrate-inhibited (2003), J. Biol. Chem., 278, 40075-40078.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.83 AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R-4-nitroanilide substrate inhibition Bacillus anthracis
3.4.24.83 AcM-L-A-R-R-R-P-V-L-P-4-nitroanilide substrate inhibition Bacillus anthracis
3.4.24.83 AcR-R-R-R-V-L-R-4-methylcoumarin-7-amide substrate inhibition Bacillus anthracis
3.4.24.83 AcR-R-R-R-V-L-R-4-nitroanilide substrate inhibition Bacillus anthracis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.83 0.0018
-
 AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R-4-nitroanilide
-
 Bacillus anthracis
3.4.24.83 0.0095
-
 AcR-R-R-R-V-L-R-4-nitroanilide
-
 Bacillus anthracis
3.4.24.83 0.03
-
 AcM-L-A-R-R-R-P-V-L-P-4-nitroanilide
-
 Bacillus anthracis
3.4.24.83 0.082
-
 AcR-R-R-R-V-L-R-4-methylcoumarin-7-amide
-
 Bacillus anthracis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.83 mitogen-activated protein kinase + H2O Bacillus anthracis cleavage within N-terminus of MAPKKs ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.83 Bacillus anthracis
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.24.83 Preferred amino acids around the cleavage site can be denoted BBBBxHx-/-H, in which B denotes Arg or Lys, H denotes a hydrophobic amino acid, and x is any amino acid. The only known protein substrates are mitogen-activated protein (MAP) kinase kinases mechanism Bacillus anthracis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.83 AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R-4-nitroanilide + H2O
-
 Bacillus anthracis AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R + 4-nitroaniline
-
 ?
3.4.24.83 AcM-L-A-R-R-R-P-V-L-P-4-nitroanilide + H2O
-
 Bacillus anthracis AcM-L-A-R-R-R-P-V-L-P + 4-nitroaniline
-
 ?
3.4.24.83 AcR-R-R-R-V-L-R-4-methylcoumarin-7-amide + H2O
-
 Bacillus anthracis AcR-R-R-R-V-L-R + 7-amino-4-methylcoumarin
-
 ?
3.4.24.83 AcR-R-R-R-V-L-R-4-nitroanilide + H2O
-
 Bacillus anthracis AcR-R-R-R-V-L-R + 4-nitroaniline
-
 ?
3.4.24.83 mitogen-activated protein kinase + H2O cleavage within N-terminus of MAPKKs Bacillus anthracis ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.24.83 37
-
-
 Bacillus anthracis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.24.83 7.4
-
 assay at Bacillus anthracis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.4.24.83 0.036
-
 AcG-Y-betaA-R-R-R-A-R-R-R-R-V-L-R-4-nitroanilide
-
 Bacillus anthracis
3.4.24.83 0.17
-
 AcR-R-R-R-V-L-R-4-methylcoumarin-7-amide
-
 Bacillus anthracis
3.4.24.83 0.19
-
 AcR-R-R-R-V-L-R-4-nitroanilide
-
 Bacillus anthracis
3.4.24.83 0.6
-
 AcM-L-A-R-R-R-P-V-L-P-4-nitroanilide
-
 Bacillus anthracis