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Literature summary extracted from

  • Sugimoto, K.; Takahashi, M.; Yamamoto, Y.; Shimada, K.; Tanzawa, K.
    Identification of aggrecanase activity in medium of cartilage culture (1999), J. Biochem., 126, 449-455.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.24.82 actinonin 0.01 mg/ml, 61% inhibition Bos taurus
3.4.24.82 AG3340 0.00066 mM, 50% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
3.4.24.82 BB94 0.000524 mM, 85% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
3.4.24.82 chondroitin sulfate
-
 Bos taurus
3.4.24.82 CT-1746 0.000048 mM, 50% inhibition, hydroxamate-based matrix metalloproteinase inhibitor Bos taurus
3.4.24.82 EDTA 5 mM, complete inhibition Bos taurus
3.4.24.82 EGTA 5 mM, complete inhibition Bos taurus
3.4.24.82 glycosaminoglycan
-
 Bos taurus
3.4.24.82 heparin
-
 Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
3.4.24.82 Ca2+ Ca2+-dependent metalloproteinase Bos taurus

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.82 Bos taurus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.82 Q Sepharose, Zn-chelating, ether Toyopearl, Mono S Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.82 articular cartilage
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.24.82 additional information
-
 5300.0 mg/h/mg protein Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.82 aggrecan + H2O Glu373-Ala374 is the major cleavage site, aggrecanase also cleaves at Glu1971-Leu1972, which is located in the gap region in the chondroitin sulfate attachment region, aggrecanase does not cleave at the matrix metalloproteinase site Asn341-Phe342 Bos taurus fragments of aggrecan
-
 ?