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Literature summary extracted from

  • Mattern-Dogru, E.; Ma, X.; Hartmann, J.; Decker, H.; Stockigt, J.
    Potential active-site residues in polyneuridine aldehyde esterase, a central enzyme of indole alkaloid biosynthesis, by modelling and site-directed mutagenesis (2002), Eur. J. Biochem., 269, 2889-2896.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.78 expressed in Escherichia coli M15 Rauvolfia serpentina Benth. ex Kurz

Protein Variants

EC Number Protein Variants Comment Organism
3.1.1.78 C132A 755% lower activity than wild-type Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 C170A 80% lower activity than wild-type Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 C213S/G152Q 35% lower activity than wild-type Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 C257A 35% lower activity than wild-type Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 H17A 97% lower activity than wild-type Rauvolfia serpentina Benth. ex Kurz

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.1.78 diethyldicarbonate 100% inhibition at 0.8 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 HgCl2 100% inhibition at 0.2 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 L-chloro-3-[4-tosyl-amido]-7-amido-2-heptanone 12% inhibition at 0.12 mM Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 phenylmethanesulfonyl fluoride 20% inhibition at 1 mM Rauvolfia serpentina Benth. ex Kurz

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.78 30000
-
 2 * 30000, SDS-PAGE Rauvolfia serpentina Benth. ex Kurz
3.1.1.78 60000
-
 gel filtration Rauvolfia serpentina Benth. ex Kurz

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.1.1.78 polyneuridine aldehyde + H2O Rauvolfia serpentina Benth. ex Kurz central enzyme of indole alkaloid biosynthesis 16-epivellosimine + CO2 + methanol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.78 Rauvolfia serpentina Benth. ex Kurz
-
 Ser87, Asp216 and His244 form a catalytic triad of polyneuridine aldehyde esterase
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.78
-
 Rauvolfia serpentina Benth. ex Kurz

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.78 polyneuridine aldehyde + H2O
-
 Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol
-
 ?
3.1.1.78 polyneuridine aldehyde + H2O central enzyme of indole alkaloid biosynthesis Rauvolfia serpentina Benth. ex Kurz 16-epivellosimine + CO2 + methanol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.78 homodimer 2 * 30000, SDS-PAGE Rauvolfia serpentina Benth. ex Kurz

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.78 30
-
 assay conditions Rauvolfia serpentina Benth. ex Kurz

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.78 8
-
 assay conditions Rauvolfia serpentina Benth. ex Kurz