Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Dignam, S.S.; Setlow, P.
    Bacillus megaterium spore protease. Action of the enzyme on peptides containing the amino acid sequence cleaved by the enzyme in vivo (1980), J. Biol. Chem., 255, 8408-8412.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.24.78 0.0014
-
 spore protein A spore protein A, Km lower than 0.014 mM Priestia megaterium
3.4.24.78 0.008
-
 spore protein B Km lower than 0.008 mM Priestia megaterium
3.4.24.78 0.4
-
 N-acetyl-Phe-Glu-Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg pH 7.4, 37°C Priestia megaterium
3.4.24.78 20
-
 N-acetyl-Thr-Glu-Phe-Ala-Ser-Glu-Phe pH 7.4, 37°C Priestia megaterium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.24.78 spore protein A + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein A
-
 ?
3.4.24.78 spore protein A + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein A
-
 ?
3.4.24.78 spore protein B + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein B
-
 ?
3.4.24.78 spore protein B + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein B
-
 ?
3.4.24.78 spore protein C + H2O Priestia megaterium specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein C
-
 ?
3.4.24.78 spore protein C + H2O Priestia megaterium QN 8155 specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond fragments of spore protein C
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.24.78 Priestia megaterium
-
-
-
3.4.24.78 Priestia megaterium QN 8155
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.24.78
-
 Priestia megaterium

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.24.78 spore
-
 Priestia megaterium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.24.78 N-acetyl-Phe-Glu-Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg + H2O
-
 Priestia megaterium N-acetyl-Phe-Glu + Ile-Ala-Ser-Glu-Phe-Gly-Val-Asn-Leu-Gly-Pro-Asp-Ala-Thr-Ala-Arg
-
 ?
3.4.24.78 N-acetyl-Thr-Glu-Phe-Ala + H2O
-
 Priestia megaterium N-acetyl-Thr-Glu + Phe-Ala
-
 ?
3.4.24.78 N-acetyl-Thr-Glu-Phe-Ala-Ser-Glu-Phe + H2O
-
 Priestia megaterium N-acetyl-Thr-Glu + Phe-Ala-Ser-Glu-Phe
-
 ?
3.4.24.78 spore protein A + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein A
-
 ?
3.4.24.78 spore protein A + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein A
-
 ?
3.4.24.78 spore protein B + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein B
-
 ?
3.4.24.78 spore protein B + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein B
-
 ?
3.4.24.78 spore protein C + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium fragments of spore protein C
-
 ?
3.4.24.78 spore protein C + H2O specific sequence of cleavage site, R-Glu-(Phe,Ile)-Ala,Gly-Ser-Glu-R, gpr endopeptidase cleaves at the first glutamyl bond Priestia megaterium QN 8155 fragments of spore protein C
-
 ?