EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.37 | 2-mercaptoethanol | - |
Porphyromonas gingivalis | |
3.4.22.37 | cysteine | - |
Porphyromonas gingivalis | |
3.4.22.37 | dithiothreitol | - |
Porphyromonas gingivalis | |
3.4.22.37 | glycyl-glycine | activates only RGP-B | Porphyromonas gingivalis | |
3.4.22.47 | 2-mercaptoethanol | 30 mM, 197% increase in activity | Porphyromonas gingivalis | |
3.4.22.47 | cysteine | 3 mM, 166% increase in activity | Porphyromonas gingivalis | |
3.4.22.47 | dithiothreitol | 30 mM, 315% increase in activity | Porphyromonas gingivalis | |
3.4.22.47 | EDTA | 2 mM, 79% increase in activity | Porphyromonas gingivalis | |
3.4.22.47 | EGTA | 2 mM, 79% increase in activity | Porphyromonas gingivalis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.37 | antipain | both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | EDTA | both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | L-trans-epoxy-succinylleucylamido-(4-guanidino)butane | both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | leupeptin | both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | additional information | no inhibition by aprotinin, epsilon-aminocaproic acid and diisopropylfluorophosphate | Porphyromonas gingivalis | |
3.4.22.37 | N-ethylmaleimide | both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | p-hydroxymercuribenzoate | complete inhibition at 0.2 mM, both enzyme forms | Porphyromonas gingivalis | |
3.4.22.37 | tosyl-L-lysine chloromethyl ketone | i.e. TLCK, both enzyme forms | Porphyromonas gingivalis | |
3.4.22.47 | N-ethylmaleimide | 2 mM, 65% inhibition | Porphyromonas gingivalis | |
3.4.22.47 | p-hydroxymercuribenzoate | 0.2 mM, 68% inhibition | Porphyromonas gingivalis | |
3.4.22.47 | tosyl-L-lysine chloromethyl ketone | 0.05 mM, 81% inhibition | Porphyromonas gingivalis |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
3.4.22.37 | cell envelope | - |
Porphyromonas gingivalis | 30313 | - |
3.4.22.37 | extracellular | - |
Porphyromonas gingivalis | - |
- |
3.4.22.47 | cell envelope | - |
Porphyromonas gingivalis | 30313 | - |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
3.4.22.37 | additional information | enzyme forms are not affected by Mg2+ and Ca2+ | Porphyromonas gingivalis |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 43000 | - |
x * 43000, both enzyme forms, SDS-PAGE | Porphyromonas gingivalis |
3.4.22.47 | 48000 | - |
x * 48000, SDS-PAGE | Porphyromonas gingivalis |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.37 | protein + H2O | Porphyromonas gingivalis | - |
peptides | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.37 | Porphyromonas gingivalis | - |
strain ATCC 33277, 2 arginine-specific gingipains RGP-A and RGP-B | - |
3.4.22.47 | Porphyromonas gingivalis | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
3.4.22.37 | RGP-A and RGP-B, from envelope material, solubilization by detergent 3-[(3-cholamidopropyl)-dimethylammonio]-1-propanesulfonate, i.e. CHAPS | Porphyromonas gingivalis |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
3.4.22.47 | culture supernatant | - |
Porphyromonas gingivalis | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.37 | benzoyl-L-arginine-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | benzoyl-L-arginine + 4-nitroaniline | - |
? | |
3.4.22.37 | benzoyl-Phe-Val-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | benzoyl-Phe-Val-Arg + 4-nitroaniline | - |
? | |
3.4.22.37 | benzoyl-Pro-Phe-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | benzoyl-Pro-Phe-Arg + 4-nitroaniline | - |
? | |
3.4.22.37 | benzoyl-Val-Gly-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | benzoyl-Val-Gly-Arg + 4-nitroaniline | - |
? | |
3.4.22.37 | butoxy-carbonyl-O-benzyl-Ser-Gly-Arg-4-nitroanilide + H2O | best sythetic substrate | Porphyromonas gingivalis | butoxy-carbonyl-O-benzyl-Ser-Gly-Arg + 4-nitroaniline | - |
? | |
3.4.22.37 | butoxy-carbonyl-Val-Leu-Gly-Arg-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | butoxy-carbonyl-Val-Leu-Gly-Arg + 4-nitroaniline | - |
? | |
3.4.22.37 | haemoglobin + H2O | complete digestion, enzyme form RGP-B shows high activity, not RGP-A, human substrate | Porphyromonas gingivalis | ? | - |
? | |
3.4.22.37 | additional information | no activity of both enzyme forms with toluenesulfonyl-glycyl-L-prolyl-L-lysine-4-nitroanilide, Val-Leu-Lys-4-nitroanilide, benzoyl-Lys-4-nitroanilide, succinyl-Ala-Ala-Pro-Phe-4-nitroanilide, glutaryl-Phe-4-nitroanilide, benzoyl-Tyr-4-nitroanilide, and Lys-4-nitroanilide, activity with chromogenic synthetic substrates is limited to those with arginine in the P1-position | Porphyromonas gingivalis | ? | - |
? | |
3.4.22.37 | protein + H2O | - |
Porphyromonas gingivalis | peptides | - |
? | |
3.4.22.37 | toluenesulfonyl-glycyl-L-prolyl-L-arginine-4-nitroanilide + H2O | - |
Porphyromonas gingivalis | toluenesulfonyl-glycyl-L-prolyl-L-arginine + 4-nitroaniline | - |
? | |
3.4.22.47 | benzyloxycarbonyl-L-Lys-p-nitroanilide + H2O | 7% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | Porphyromonas gingivalis | benzyloxycarbonyl-L-Lys + p-nitroaniline + H2O | - |
? | |
3.4.22.47 | D-Val-Phe-Lys-p-nitroanilide + H2O | 70% of activity with N-p-tosyl-Gly-Pro-Lys-p-nitroanilide | Porphyromonas gingivalis | D-Val-Phe-Lys + p-nitroaniline | - |
? | |
3.4.22.47 | N-p-tosyl-Gly-Pro-Lys-p-nitroanilide + H2O | - |
Porphyromonas gingivalis | N-p-tosyl-Gly-Pro-Lys + p-nitroaniline | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
3.4.22.37 | ? | x * 43000, both enzyme forms, SDS-PAGE | Porphyromonas gingivalis |
3.4.22.47 | ? | x * 48000, SDS-PAGE | Porphyromonas gingivalis |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
3.4.22.37 | Arg-gingipain | - |
Porphyromonas gingivalis |
3.4.22.37 | arginine-gingipain | - |
Porphyromonas gingivalis |
3.4.22.37 | Arginine-specific gingipain | - |
Porphyromonas gingivalis |
3.4.22.37 | RGP | - |
Porphyromonas gingivalis |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 37 | - |
assay at, substrate haemoglobin | Porphyromonas gingivalis |
EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 45 | - |
50 mM Tris,-HCl, pH 7.8, 10 min, remaining activity for RGP-A is 82% and for RGP-B 52% of maximal activity, after 30 min remaining activity for RGP-A is 58% and for RGP-B 26% of maximal activity | Porphyromonas gingivalis |
3.4.22.47 | 45 | - |
68% loss of activity after 10 min, 97% loss of activity after 30 min | Porphyromonas gingivalis |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 7.5 | 8 | both enzyme forms | Porphyromonas gingivalis |
3.4.22.47 | 7.5 | - |
- |
Porphyromonas gingivalis |
EC Number | pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.37 | 8.5 | - |
rapid decrease of activity above pH 8.5 | Porphyromonas gingivalis |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
3.4.22.37 | Porphyromonas gingivalis | RGP-B, isoelectric focusing | - |
3.8 |
3.4.22.37 | Porphyromonas gingivalis | RGP-A, isoelectric focusing | 4.8 | 4.7 |