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Literature summary extracted from

  • Yamamoto, K.; Kamata, O.; Kato, Y.
    Separation and properties of three forms of cathepsin H-like cysteine proteinase from rat spleen (1984), J. Biochem., 95, 477-484.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.16 2-mercaptoethanol activates Rattus norvegicus
3.4.22.16 cysteine activates Rattus norvegicus
3.4.22.16 dithiothreitol activates Rattus norvegicus
3.4.22.16 glutathione activates Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.4.22.16 ethyl(2S,3S)-3-(S)-3-methyl-1-(3-methylbutylcarbamoyl)butylcarbamoyl oxirane-2-carboxylate 65-76% inhibition at 0.01 mg/ml, isoform-dependent Rattus norvegicus
3.4.22.16 iodoacetic acid 71-77% inhibition at 0.5 mM, isoenzyme-dependent Rattus norvegicus
3.4.22.16 tosyl-Leu-CH2Cl 17-35% inhibition at 0.1 mM, isoenzyme-dependent Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 22000
-
 gel filtration, isoform 3 Rattus norvegicus
3.4.22.16 26000
-
 gel filtration, isoform 2 Rattus norvegicus
3.4.22.16 28000
-
 gel filtration, isoform 1 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Rattus norvegicus
-
 three isoforms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.16
-
 Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.16 spleen
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.16 7.714
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
 Rattus norvegicus alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Rattus norvegicus Leu + 2-naphthylamine
-
 ir

Synonyms

EC Number Synonyms Comment Organism
3.4.22.16 cathepsin H-like cysteine proteinase
-
-

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 6.5
-
 isoforms 1 and 3 Rattus norvegicus
3.4.22.16 7.5
-
 isoform 2 Rattus norvegicus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.16 5 9
-
 Rattus norvegicus