EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.16 | additional information | - |
additional information | pH and temperature dependence of kcat/Km | Rattus norvegicus | |
3.4.22.16 | 0.076 | - |
citrulline-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus | |
3.4.22.16 | 0.097 | - |
Arg-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus | |
3.4.22.16 | 0.321 | - |
benzoyl-Arg-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
3.4.22.16 | Rattus norvegicus | - |
- |
- |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
3.4.22.16 | Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-/- bonds) as well as an endopeptidase | mechanism: specific interactions of an anionic active site residue with the charged alpha-amino group of substrates cause transition state stabilization which proves the enzyme to act preferentially as an aminopeptidase | Rattus norvegicus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
3.4.22.16 | Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | Arg + 7-amino-4-methylcoumarin | - |
ir | |
3.4.22.16 | benzoyl-Arg-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | benzoyl-Arg + 7-amino-4-methylcoumarin | - |
ir | |
3.4.22.16 | citrulline-4-methylcoumaryl-7-amide + H2O | - |
Rattus norvegicus | citrulline + 7-amino-4-methylcoumarin | - |
ir |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
3.4.22.16 | additional information | - |
additional information | pH and temperature dependence of kcat/Km | Rattus norvegicus | |
3.4.22.16 | 0.63 | - |
benzoyl-Arg-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus | |
3.4.22.16 | 3.1 | - |
citrulline-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus | |
3.4.22.16 | 8.13 | - |
Arg-4-methylcoumaryl-7-amide | at 30°C | Rattus norvegicus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
3.4.22.16 | 6.5 | - |
substrates Arg-4-methylcoumaryl-7-amide and citrulline-4-methylcoumaryl-7-amide | Rattus norvegicus |
3.4.22.16 | 8 | - |
or higher, substrate benzoyl-Arg-4-methylcoumaryl-7-amide | Rattus norvegicus |