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Literature summary extracted from

  • Aranishi, F.; Hara, K.; Osatomi, K.; Ishihara, T.
    Substrate specificity of carp Cyprinus carpio cathepsin H with methylcoumarylamide substrates (1997), Comp. Biochem. Physiol. B, 116B, 203-208.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 35.4
-
 Leu-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 72
-
 Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 184
-
 Lys-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 210
-
 Ala-4-methylcoumaryl-7-amide
-
 Cyprinus carpio

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 29000
-
 gel filtration Cyprinus carpio

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.16 Proteins + H2O Cyprinus carpio aminopeptidase and endopeptidase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Cyprinus carpio
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.16
-
 Cyprinus carpio

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.16 hepatopancreas
-
 Cyprinus carpio
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.16 29.7
-
-
 Cyprinus carpio

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 Ala-4-methylcoumaryl-7-amide + H2O
-
 Cyprinus carpio Ala + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 Arg-4-methylcoumaryl-7-amide + H2O
-
 Cyprinus carpio Arg + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 Leu-4-methylcoumaryl-7-amide + H2O
-
 Cyprinus carpio Leu + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 Lys-4-methylcoumaryl-7-amide + H2O
-
 Cyprinus carpio Lys + 7-amino-4-methylcoumarin
-
 ir
3.4.22.16 Proteins + H2O aminopeptidase and endopeptidase activity Cyprinus carpio ?
-
 ?

Subunits

EC Number Subunits Comment Organism
3.4.22.16 dimer 1 * 29000, 1* 4000, SDS-PAGE, partial Cyprinus carpio
3.4.22.16 monomer 1* 29000, SDS-PAGE, partial Cyprinus carpio

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 38
-
 assay at Cyprinus carpio

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.16 1.1
-
 Leu-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 1.87
-
 Ala-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 2.2
-
 Arg-4-methylcoumaryl-7-amide
-
 Cyprinus carpio
3.4.22.16 4.13
-
 Lys-4-methylcoumaryl-7-amide
-
 Cyprinus carpio

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 6.5 7.2 substrate-dependent Cyprinus carpio