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Literature summary extracted from

  • Singh, H.; Kalnitsky, G.
    Separation of a new alpha-N-benzoylarginine-beta-naphthylamide hydrolase from cathepsin B1 (1978), J. Biol. Chem., 253, 4319-4326.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.4.22.16 EDTA required Oryctolagus cuniculus
3.4.22.16 thiol compounds required Oryctolagus cuniculus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.4.22.16 26000
-
 gel filtration Oryctolagus cuniculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.16 Proteins + H2O Oryctolagus cuniculus aminopeptidase and endopeptidase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Oryctolagus cuniculus
-
 6 isoenzymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.4.22.16
-
 Oryctolagus cuniculus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.4.22.16 lung
-
 Oryctolagus cuniculus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.4.22.16 additional information
-
-
 Oryctolagus cuniculus
3.4.22.16 7.45
-
-
 Oryctolagus cuniculus

Storage Stability

EC Number Storage Stability Organism
3.4.22.16 4°C, 50 mM Na acetate buffer, pH 4.6, 1 mM EDTA, 1 month, concentrated preparations, no loss of activity Oryctolagus cuniculus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 alpha-N-benzoyl-DL-arginine-beta-naphthylamide + H2O
-
 Oryctolagus cuniculus alpha-N-benzoyl-DL-arginine + beta-naphthylamine
-
 ir
3.4.22.16 Leu-2-naphthylamide + H2O
-
 Oryctolagus cuniculus Leu + 2-naphthylamine
-
 ir
3.4.22.16 Proteins + H2O aminopeptidase and endopeptidase activity Oryctolagus cuniculus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.4.22.16 alpha-N-benzoylarginine-beta-naphthylamide hydrolase
-
 Oryctolagus cuniculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 38
-
 substrate Leu-2-naphthylamide Oryctolagus cuniculus
3.4.22.16 49
-
 substrate benzoyl-Arg 2-naphthylamide Oryctolagus cuniculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 6.5
-
 substrate benzoyl-Arg 2-naphthylamide Oryctolagus cuniculus
3.4.22.16 7.2
-
 substrate Leu-2-naphthylamide Oryctolagus cuniculus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.4.22.16 additional information
-
 little sensitivity to alkaline pH Oryctolagus cuniculus
3.4.22.16 8.5
-
 83% activity Oryctolagus cuniculus