Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Brümme, D.; Bescherer, K.; Kirschke, H.; Fittkau, S.
    Enzyme-substrate interactions in the hydrolysis of peptides by cathepsins B and H from rat liver (1987), Biochem. J., 245, 381-385.
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.4.22.16 0.7
-
 succinyl-Ala-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.7
-
 succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 2.9
-
 succinyl-Pro-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 3
-
 succinyl-Ala-Pro-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 3.5
-
 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2
-
 Rattus norvegicus
3.4.22.16 7
-
 succinyl-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 7.4
-
 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro
-
 Rattus norvegicus
3.4.22.16 10.2
-
 succinyl-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 27.8
-
 succinyl-Ala-Ala-Pro-4-nitroanilide
-
 Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.4.22.16 Proteins + H2O Rattus norvegicus rather broad specificity of aminopeptidase activity ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.4.22.16 Rattus norvegicus
-
-
-

Reaction

EC Number Reaction Comment Organism Reaction ID
3.4.22.16 Hydrolysis of proteins, acting as an aminopeptidase (notably, cleaving Arg-/- bonds) as well as an endopeptidase number of possible subsites: three Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.4.22.16 acetyl-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus acetyl-Ala + 4-nitroaniline
-
 ir
3.4.22.16 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Pro-Ala-Ala-Ala + Pro
-
 ir
3.4.22.16 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2 + H2O
-
 Rattus norvegicus benzyloxycarbonyl-Pro-Ala-Ala-Ala + Pro-NH2
-
 ir
3.4.22.16 additional information influence of Pro in position P1 to P3 on rate of peptide hydrolysis, P3 i.e. 3rd amino acid in N-terminal direction relative to point of cleavage, greatest increase in activity after substrate elongation to tetrapeptide Rattus norvegicus ?
-
 ?
3.4.22.16 Proteins + H2O rather broad specificity of aminopeptidase activity Rattus norvegicus ?
-
 ?
3.4.22.16 succinyl-Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Ala + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Ala-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Ala-Ala + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Ala-Ala-Ala + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Ala-Ala-Pro-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Ala-Pro + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Ala-Pro-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Ala-Pro-Ala + 4-nitroaniline
-
 ir
3.4.22.16 succinyl-Pro-Ala-Ala-4-nitroanilide + H2O
-
 Rattus norvegicus succinyl-Pro-Ala-Ala + 4-nitroaniline
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.4.22.16 40
-
 assay at Rattus norvegicus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.4.22.16 0.004
-
 succinyl-Ala-Ala-Pro-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.07
-
 succinyl-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.15
-
 succinyl-Pro-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.3
-
 succinyl-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.8
-
 succinyl-Ala-Ala-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 0.9
-
 succinyl-Ala-Ala-Ala-Ala-4-nitroanilide
-
 Rattus norvegicus
3.4.22.16 5.3
-
 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro
-
 Rattus norvegicus
3.4.22.16 7.7
-
 benzyloxycarbonyl-Pro-Ala-Ala-Ala-Pro-NH2
-
 Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.4.22.16 6.5
-
 assay at Rattus norvegicus