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Literature summary extracted from

  • Burczak, J.D.; Fairley, J.L.; Sweeley, C.C.
    Characterization of a CMP-sialic acid:lactosylceramide sialyltransferase activity in cultured hamster cells (1984), Biochim. Biophys. Acta, 804, 442-449.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.9 Cutscum
-
 Mesocricetus auratus
2.4.3.9 additional information not activated by Tween 20 or 80 Mesocricetus auratus
2.4.3.9 Triton CF-54 activation, most potent activator Mesocricetus auratus
2.4.3.9 Triton CF-54/Tween 80
-
 Mesocricetus auratus
2.4.3.9 Triton X-100 activation Mesocricetus auratus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 asialofetuin weak Mesocricetus auratus
2.4.3.9 ganglioside GM3 0.5 mM and 1 mM, 43% and 60% inhibition respectively Mesocricetus auratus
2.4.3.9 Globotetraosylceramide weak Mesocricetus auratus
2.4.3.9 ionic detergents
-
 Mesocricetus auratus
2.4.3.9 additional information glucosylceramide or globotriaosylceramide Mesocricetus auratus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.9 0.11
-
 lactosylceramide
-
 Mesocricetus auratus
2.4.3.9 0.16
-
 CMP-N-acetylneuraminate
-
 Mesocricetus auratus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.9 Ca2+ activation, slightly less efficient than Mn2+ Mesocricetus auratus
2.4.3.9 Mg2+ activation, slightly less efficient than Mn2+ Mesocricetus auratus
2.4.3.9 Mn2+ 15 mM, required for maximal activity Mesocricetus auratus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide Mesocricetus auratus involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Mesocricetus auratus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 cell suspension culture
-
 Mesocricetus auratus
-
2.4.3.9 fibroblast contact-inhibited cell-line NIL-8 Mesocricetus auratus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Mesocricetus auratus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.9 CMP-N-acetylneuraminate + lactosylceramide involved in sequential addition of monosaccharides from sugar nucleotides to non-reducing end of oligosaccharide chains of glycosphingolipids Mesocricetus auratus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.9 additional information
-
 no effects of buffer on activity Mesocricetus auratus
2.4.3.9 6.5
-
 broad optimum in cacodylate-HCl buffer Mesocricetus auratus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.9 4.5 8
-
 Mesocricetus auratus