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Literature summary extracted from

  • Melkerson-Watson, L.J.; Sweeley, C.C.
    Special considerations in the purification of the GM3 ganglioside forming enzyme, CMP-sialic acid:lactosylceramide alpha 2-3 sialyltransferase (SAT-1): solubilization of SAT-1 with lauryldimethylamine oxide (1990), Biochem. Biophys. Res. Commun., 172, 165-171.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.9 beta-octylglucoside
-
 Rattus norvegicus
2.4.3.9 Lauryldimethylamine oxide i.e. Ammonyx LO, nonionic/cationic detergent, 12-15fold higher activation than by Triton CF-54, Triton X-100 or beta-octylglucoside Rattus norvegicus
2.4.3.9 Triton CF-54
-
 Rattus norvegicus
2.4.3.9 Triton X-100 activation Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.3.9 lauryldimethylamine oxide solubilizes and stabilizes solubilized enzyme during purification and storage Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.9 60000
-
 x * 60000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 liver
-
 Rattus norvegicus
-

Storage Stability

EC Number Storage Stability Organism
2.4.3.9 -80°C, 25 mM sodium cacodylate, pH 6.5, 15% w/v lauryldimethylamine oxide, 6-12 months, no loss of activity Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.9 ? x * 60000, SDS-PAGE Rattus norvegicus