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Literature summary extracted from

  • Melkerson-Watson, L.J.; Sweeley, C.C.
    Special considerations in the purification of the GM3 ganglioside-forming enzyme, CMP-sialic acid:lactosylceramide alpha 2-3 sialyltransferase (SAT-1): effects of protease inhibitors on rat hepatic SAT-1 activity (1991), Biochem. Biophys. Res. Commun., 175, 325-332.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.3.9 2-mercaptoethanol 0.18 mM, 392% activation of purified sialyltransferase-1, 1120% activation of sialytransferase-1 activity in microsomes Rattus norvegicus

General Stability

EC Number General Stability Organism
2.4.3.9 PMSF, leupeptin or pepstatin A stabilize during purification Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 (4-Amidinophenyl)-methanesulfonyl fluoride serine protease, 0.02 mM, 48% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 alpha2-Macroglobulin 1 unit, 67% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes Rattus norvegicus
2.4.3.9 Aprotinin serine protease inhibitor, 0.0003 mM, 74% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 dithiothreitol 0.1 mM, 89% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 E-64 thiol protease inhibitor, 0.0028 mM, 71% inhibition of purified sialyltransferase-1, activation of sialytransferase-1 activity in microsomes Rattus norvegicus
2.4.3.9 EDTA 0.1 mM, 71% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 Ep-475 thiol protease inhibitor, 0.0028 mM, 75% inhibition of purified sialyltransferase-1; thiol protease inhibitor, 0.0028 mM, 84% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 L-1-chloro-3-[4-tosylamido]-4-phenyl-2-butanone chymotrypsin and thiol protease inhibitor, 0.284 mM, 67% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 L-1-Chloro-3-[4-tosylamido]-7-amino-2-heptanone-HCl trypsin and thiol protease inhibitor, 0.135 mM, 73% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 leupeptin serine and thiol protease inhibitor, 0.001 mM, 72% inhibition of purified sialyltransferase-1, no inhibition of sialyltransferase-1 activity in microsomes Rattus norvegicus
2.4.3.9 additional information inhibition in vivo i.e. stable microsomes not as dramatic as in vitro Rattus norvegicus
2.4.3.9 N3- 1 mM, 87% inhibition of purified sialyltransferase-1 Rattus norvegicus
2.4.3.9 pepstatin A 0.001 mM, 72% inhibition of purified sialyltransferase-1, activation of sialyltransferase-1 activity in microsomes Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.9 Golgi apparatus
-
 Rattus norvegicus 5794
-
2.4.3.9 microsome
-
 Rattus norvegicus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.9 Mn2+
-
 Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Rattus norvegicus
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide
-
 Rattus norvegicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?