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Literature summary extracted from

  • Melkerson-Watson, L.J.; Sweeley, C.C.
    Purification to apparent homogeneity by immunoaffinity chromatography and partial characterization of the GM3 ganglioside-forming enzyme, CMP-sialic acid:lactosylceramide alpha 2,3-sialyltransferase (SAT-1), from rat liver Golgi [published erratum appears in J Biol Chem 1991 Oct 15;266(29):19865] (1991), J. Biol. Chem., 266, 4448-4457.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.3.9 CDP
-
 Rattus norvegicus
2.4.3.9 CMP
-
 Rattus norvegicus
2.4.3.9 CTP
-
 Rattus norvegicus
2.4.3.9 Monoclonal antibody M12GC7
-
 Rattus norvegicus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.3.9 0.00011
-
 lactosylceramide
-
 Rattus norvegicus
2.4.3.9 0.00026
-
 CMP-N-acetylneuraminate
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.3.9 Golgi apparatus
-
 Rattus norvegicus 5794
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.3.9 Mn2+
-
 Rattus norvegicus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.3.9 60000
-
 x * 60000, SDS-PAGE Rattus norvegicus

Organism

EC Number Organism UniProt Comment Textmining
2.4.3.9 Rattus norvegicus
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.3.9 glycoprotein N- and O-linked carbohydrate side chains, containing sialic acids in alpha-2,3 and 2,6-linkage, galactose and galactosamine, branched N-glycans containing mannose Rattus norvegicus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.3.9 liver
-
 Rattus norvegicus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.3.9 0.0055
-
-
 Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,3-N-acetylgalactosamin-1,4-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. asialo-ganglioside GM1, sialylated at about 84% the rate of lactosylceramide Rattus norvegicus CMP + ?
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide preferred acceptors have the general structure beta1-O-ceramide, disaccharides are preferred to monosaccharide Rattus norvegicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.9 CMP-N-acetylneuraminate + beta-D-galactosyl-1,4-beta-D-glucosylceramide no acceptors are fetuin, mucin, alpha1-acid glycoprotein, glycophorin or their respective asialo-derivatives, gangliosides GD1b, GM3, GM2, GM1 or GD1a Rattus norvegicus CMP + N-acetylneuraminyl-2,3-beta-D-galactosyl-1,4-beta-D-glucosylceramide i.e. ganglioside GM3 ?
2.4.3.9 CMP-N-acetylneuraminate + galactosylceramide sialylated at about 40% the rate of lactosylceramide Rattus norvegicus CMP + ?
-
 ?
2.4.3.9 CMP-N-acetylneuraminate + glucosylceramide sialylated at about 65% the rate of lactosylceramide Rattus norvegicus CMP + ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.3.9 ? x * 60000, SDS-PAGE Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.3.9 additional information
-
 pI: 5.7-6.2 Rattus norvegicus
2.4.3.9 6.5
-
-
 Rattus norvegicus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.3.9 6.1 7.6 detectable activity Rattus norvegicus