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Literature summary extracted from

  • Laskaris, G.; van der Heijden, R.; Verpoorte, R.
    Purification and partial characterization of geranylgeranyl diphosphate synthase, from Taxus baccata cell cultures. An enzyme that regulates taxane biosynthesis (2000), Plant Sci., 153, 97-105.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.29 0.0005
-
farnesyl diphosphate
-
Ricinus communis
2.5.1.29 0.0015
-
isopentenyl diphosphate
-
Taxus baccata
2.5.1.29 0.00246
-
farnesyl diphosphate
-
Taxus baccata
2.5.1.29 0.0035
-
isopentenyl diphosphate
-
Ricinus communis
2.5.1.29 0.0127
-
dimethylallyl diphosphate
-
Taxus baccata

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.29 additional information enzyme seems to be membrane associated Taxus baccata
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.5.1.29 Mg2+ required for activity Taxus baccata
2.5.1.29 Mg2+ maximal activity at 2 mM together with Mn2+ at 3 mM Taxus baccata
2.5.1.29 Mn2+ required for activity Taxus baccata
2.5.1.29 Mn2+ most effective divalent cation, maximal activity at 3 mM Taxus baccata
2.5.1.29 Zn2+ least effective divalent cation Taxus baccata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.29 38000
-
2 * 38000, SDS-PAGE Taxus baccata
2.5.1.29 72000
-
gel filtration Ricinus communis
2.5.1.29 76000
-
gel filtration Taxus baccata

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Ricinus communis no activity with dimethylallyl diphosphate geranylgeranyl diphosphate + diphosphate
-
?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate Taxus baccata enzyme regulates taxane biosynthesis geranylgeranyl diphosphate + diphosphate
-
?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate Taxus baccata low activity geranylgeranyl diphosphate + 3 diphosphate
-
?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate Taxus baccata
-
geranylgeranyl diphosphate + 2 diphosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.29 Ricinus communis
-
-
-
2.5.1.29 Taxus baccata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.29 ammonium sulfate, phenyl Sepharose 6, Mono Q, Tosohaas-G3000, Mono P Taxus baccata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.29 cell suspension culture
-
Taxus baccata
-
2.5.1.29 seedling
-
Ricinus communis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.29 13.8
-
-
Taxus baccata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate
-
Taxus baccata geranylgeranyl diphosphate + diphosphate
-
?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate no activity with dimethylallyl diphosphate Ricinus communis geranylgeranyl diphosphate + diphosphate
-
?
2.5.1.29 (E,E)-farnesyl diphosphate + isopentenyl diphosphate enzyme regulates taxane biosynthesis Taxus baccata geranylgeranyl diphosphate + diphosphate
-
?
2.5.1.29 dimethylallyl diphosphate + 3 isopentenyl diphosphate low activity Taxus baccata geranylgeranyl diphosphate + 3 diphosphate
-
?
2.5.1.29 geranyl diphosphate + 2 isopentenyl diphosphate
-
Taxus baccata geranylgeranyl diphosphate + 2 diphosphate
-
?

Subunits

EC Number Subunits Comment Organism
2.5.1.29 dimer 2 * 38000, SDS-PAGE Taxus baccata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.29 6.9 7.2
-
Taxus baccata