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Literature summary extracted from

  • Grieshaber, M.
    On the evolution of a oligocephalic enzyme. Glutamine-chorismate-amidotransferase-free anthranilate phosphoribosyltransferases from mutant strains of Salmonella typhimurium (1978), Z. Naturforsch. C, 33c, 235-244.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.18 L-tryptophan
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 N-(5-phospho-D-ribosyl)-anthranilate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 Sodium diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.18 0.003
-
 anthranilate TAX6trpR782 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 0.004
-
 anthranilate trpAB1653trpR782 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 0.013
-
 5-phospho-alpha-D-ribose 1-diphosphate TAX6trpR782 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 0.06
-
 5-phospho-alpha-D-ribose 1-diphosphate trpAB1653trpR782 Salmonella enterica subsp. enterica serovar Typhimurium

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.18 Mg2+ required Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.18 72000
-
 x * 72000, strain trpAB1653trpR782, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 220000
-
 and larger than 1000000, strain trpAB1653trpR782, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 320000
-
 strain TAX6trpR782, gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate Salmonella enterica subsp. enterica serovar Typhimurium enzyme of tryptophan biosynthesis N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.18 Salmonella enterica subsp. enterica serovar Typhimurium
-
 strains: trp+, TAX6trpR782, trpA703trpR782 and trpAB1653trpR782
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.18 enzyme complex, homogeneity Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.18 additional information
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 0.5
-
 strain TAX6trpR782 and trpAB165trpR782 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 2.7
-
 strain trpA703trpR782 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 3.2
-
 strain trp+ Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 17.6
-
 strain trpAB1653 Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?
2.4.2.18 anthranilate + 5-phospho-alpha-D-ribose 1-diphosphate enzyme of tryptophan biosynthesis Salmonella enterica subsp. enterica serovar Typhimurium N-(5-phospho-D-ribosyl)-anthranilate + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.18 ? x * 72000, strain trpAB1653trpR782, SDS-PAGE Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 More enzyme complex is a tetramer composed of 2 molecules each of component II and II Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.18 46
-
 half-life: strain TAX6trpR782: 1.5 min, strain trpAB1653trpR782: 5 min Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.18 6.9
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.18 7
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium