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Literature summary extracted from

  • Pagnussat, G.C.; Curatti, L.; Salerno, G.L.
    Rice sucrose-phosphate synthase: identification of an isoform specific for heterotrophic tissues with distinct metabolite regulation from the mature leaf enzyme (2000), Physiol. Plant., 108, 337-344.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 glucose-6-phosphate SPS-I and SPS-II Oryza sativa

General Stability

EC Number General Stability Organism
2.4.1.14 SPS-I loses more activity during purification than SPS-II Oryza sativa

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 phosphate SPS-I shows high sensitivity towards phosphate, whereas SPS-II is only slightly sensitive Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 3.5
-
D-fructose 6-phosphate SPS-I Oryza sativa
2.4.1.14 3.6
-
UDP-glucose SPS-I Oryza sativa
2.4.1.14 4.1
-
D-fructose 6-phosphate SPS-II Oryza sativa
2.4.1.14 4.2
-
UDP-glucose SPS-II Oryza sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 116000
-
SDS-PAGE, minor 88000 Da band detected, that is suggested to be a degradation product Oryza sativa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Oryza sativa catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Oryza sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II Oryza sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
Oryza sativa
-
2.4.1.14 seed germinated Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
Oryza sativa UDP + sucrose 6-phosphate
-
?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Oryza sativa UDP + sucrose 6-phosphate
-
?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.14 More two forms of enzyme identified, that differ in regulatory properties Oryza sativa