Literature summary extracted from
Salerno, G.L.; Pagnussat, G.C.; Pontis, H.G.
Studies on sucrose-phosphate synthase from rice leaves (1998), Cell. Mol. Biol., 44, 407-416.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
2.4.1.14 |
glucose-6-phosphate |
only SPS-II is activated, SPS-I not affected |
Oryza sativa |
|
2.4.1.14 |
Mg2+ |
stimulates enzyme activity |
Oryza sativa |
|
General Stability
EC Number |
General Stability |
Organism |
---|
2.4.1.14 |
SPS-I loses more activity during purification than SPS-II |
Oryza sativa |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
2.4.1.14 |
phosphate |
only SPS-II is inhibited, SPS-I not affected |
Oryza sativa |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
2.4.1.14 |
Mg2+ |
stimulates enzyme activity |
Oryza sativa |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
2.4.1.14 |
116000 |
- |
SDS-PAGE, minor 88000 Da band detected, different molecular weight for the two forms of enzyme postulated |
Oryza sativa |
2.4.1.14 |
420000 |
- |
gel filtration |
Oryza sativa |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
2.4.1.14 |
UDP-alpha-D-glucose + D-fructose 6-phosphate |
Oryza sativa |
catalyzes the penultimate step of sucrose synthesis |
UDP + sucrose 6-phosphate |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
2.4.1.14 |
Oryza sativa |
- |
rice |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
2.4.1.14 |
partial, two forms of the enzyme identified, SPS-I loses more activity during purification than SPS-II |
Oryza sativa |
Source Tissue
EC Number |
Source Tissue |
Comment |
Organism |
Textmining |
---|
2.4.1.14 |
leaf |
- |
Oryza sativa |
- |
2.4.1.14 |
additional information |
no activity in roots |
Oryza sativa |
- |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
2.4.1.14 |
2.9 |
- |
purified enzyme |
Oryza sativa |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
2.4.1.14 |
UDP-alpha-D-glucose + D-fructose 6-phosphate |
- |
Oryza sativa |
UDP + sucrose 6-phosphate |
- |
? |
|
2.4.1.14 |
UDP-alpha-D-glucose + D-fructose 6-phosphate |
catalyzes the penultimate step of sucrose synthesis |
Oryza sativa |
UDP + sucrose 6-phosphate |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
2.4.1.14 |
tetramer |
4 * 116000, gel filtration, SDS-PAGE |
Oryza sativa |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
2.4.1.14 |
More |
two forms of enzyme identified, that differ in regulatory properties |
Oryza sativa |
2.4.1.14 |
More |
SPS-I not affected by phosphate or glucose-6-phosphate |
Oryza sativa |