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Literature summary extracted from

  • Porchia, A.C.; Salerno, G.L.
    Sucrose biosynthesis in a prokaryotic organism: Presence of two sucrose-phosphate synthases in Anabaena with remarkable differences compared with the plant enzymes (1996), Proc. Natl. Acad. Sci. USA, 93, 13600-13604.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 Mg2+ required Anabaena sp.
2.4.1.14 Mn2+ required Anabaena sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 EDTA complete inhibition, can be restored by addition of Mg2+ or Mn2+ Anabaena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 0.8
-
 D-fructose 6-phosphate SPS-I Anabaena sp.
2.4.1.14 1.1
-
 D-fructose 6-phosphate SPS-II Anabaena sp.
2.4.1.14 1.3
-
 UDP-glucose SPS-I Anabaena sp.
2.4.1.14 4.3
-
 UDP-glucose SPS-II Anabaena sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 46000
-
 gel filtration Anabaena sp.

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Anabaena sp. catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Anabaena sp. 7119 catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Anabaena sp.
-
-
-
2.4.1.14 Anabaena sp. 7119
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 partial, two forms of the enzyme identified, enzyme becomes extremely unstable during the course of purification Anabaena sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.14 0.04
-
 purified SPS-I Anabaena sp.
2.4.1.14 0.25
-
 purified SPS-II Anabaena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 ADP-glucose + D-fructose 6-phosphate only SPS-I Anabaena sp. ADP + sucrose 6-phosphate
-
 ?
2.4.1.14 ADP-glucose + D-fructose 6-phosphate only SPS-I Anabaena sp. 7119 ADP + sucrose 6-phosphate
-
 ?
2.4.1.14 GDP-glucose + D-fructose 6-phosphate only SPS-I Anabaena sp. GDP + sucrose 6-phosphate
-
 ?
2.4.1.14 GDP-glucose + D-fructose 6-phosphate only SPS-I Anabaena sp. 7119 GDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Anabaena sp. UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Anabaena sp. UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Anabaena sp. 7119 UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Anabaena sp. 7119 UDP + sucrose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.14 monomer 1 * 46000, gel filtration Anabaena sp.

Synonyms

EC Number Synonyms Comment Organism
2.4.1.14 More two forms of enzyme identified, SPS-I and SPS-II differ in substrate specificity Anabaena sp.