Literature summary extracted from

Sonnewald, U.; Quick, W.P.; MacRae, E.; Krause, K.P.; Stitt, M.
Purification, cloning and expression of spinach leaf sucrose-phosphate synthase in Escherichia coli (1993), Planta, 189, 174-181.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.4.1.14	expression in Escherichia coli and tobacco, active enzyme	Spinacia oleracea

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.4.1.14	phosphate	E. coli expressed enzyme shows little sensitivity to phosphate inhibition, whereas tobacco expressed enzyme is highly phosphate sensitive	Spinacia oleracea

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
2.4.1.14	117600	-	calculated from DNA sequence	Spinacia oleracea
2.4.1.14	120000	-	SDS-PAGE	Spinacia oleracea
2.4.1.14	240000	-	native PAGE	Spinacia oleracea
2.4.1.14	480000	-	gel filtration	Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number	Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
2.4.1.14	UDP-alpha-D-glucose + D-fructose 6-phosphate	Spinacia oleracea	catalyzes the penultimate step of sucrose synthesis	UDP + sucrose 6-phosphate	-	?

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.4.1.14	Spinacia oleracea	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.4.1.14	-	Spinacia oleracea

Source Tissue

EC Number	Source Tissue	Comment	Organism	Textmining
2.4.1.14	leaf	-	Spinacia oleracea	-

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.4.1.14	79.5	-	purified enzyme	Spinacia oleracea

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.4.1.14	UDP-alpha-D-glucose + D-fructose 6-phosphate	-	Spinacia oleracea	UDP + sucrose 6-phosphate	-	?
2.4.1.14	UDP-alpha-D-glucose + D-fructose 6-phosphate	catalyzes the penultimate step of sucrose synthesis	Spinacia oleracea	UDP + sucrose 6-phosphate	-	?

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Release 2023.1 (January 2023)