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Literature summary extracted from

  • Salvucci, M.E.; Drake, R.R.; Haley, B.E.
    Purification and photoaffinity labeling of sucrose phosphate synthase from spinach leaves (1990), Arch. Biochem. Biophys., 281, 212-218.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 5-azido-UDP-glucose nearly complete inhibition at 1 mM Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 2.9
-
 UDP-glucose
-
 Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 120000
-
 SDS-PAGE Spinacia oleracea
2.4.1.14 253000
-
 sucrose density gradient centrifugation Spinacia oleracea
2.4.1.14 413000
-
 gel filtration of crude leaf extracts Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 enzyme becomes extremely unstable during the course of purification Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
 Spinacia oleracea
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.14 57
-
 purified enzyme Spinacia oleracea

Storage Stability

EC Number Storage Stability Organism
2.4.1.14 -80°C, purified enzyme stable for at least 4 months Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.14 dimer 2 * 120000, sucrose density gradient centrifugation, SDS-PAGE Spinacia oleracea