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Literature summary extracted from

  • Doehlert, D.C.; Huber, S.C.
    Spinach leaf sucrose phosphate snthase. Activation by glucose 6-phosphate and interaction with inorganic phosphate (1983), FEBS Lett., 153, 293-297.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.14 1,5-Anhydroglucitol 6-phosphate
-
 Spinacia oleracea
2.4.1.14 glucose-6-phosphate reduces Km of fructose-6-phosphate, suggested to be a metabolic regulator in vivo Spinacia oleracea

General Stability

EC Number General Stability Organism
2.4.1.14 20% ethylene glycol and 0.2 M KCl stabilize activity Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 phosphate in absence of glucose-6-phosphate, suggested to be a metabolic regulator in vivo Spinacia oleracea
2.4.1.14 sucrose-6-phosphate
-
 Spinacia oleracea
2.4.1.14 UDP
-
 Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 1.4
-
 D-fructose 6-phosphate in presence of 2.5 mM glucose 6-phosphate Spinacia oleracea
2.4.1.14 1.9
-
 UDP-glucose in presence and absence of glucose 6-phosphate Spinacia oleracea
2.4.1.14 4.1
-
 D-fructose 6-phosphate in the absence of glucose 6-phosphate Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 gel filtration is carried out in presence of 20% ethylene glycol and 0.2 M KCl to stabilize activity Spinacia oleracea
2.4.1.14 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
 Spinacia oleracea
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate
-
 Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
 ?