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Literature summary extracted from

  • Harbron, S.; Foyer, C.; Walker, D.
    The purification and properties of sucrose-phosphate synthetase from spinach leaves: the involvement of this enzyme and fructose bisphosphatase in the regulation of sucrose biosynthesis (1981), Arch. Biochem. Biophys., 212, 237-246.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.14 sensitive to freezing and thawing Spinacia oleracea

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.14 ADP
-
Spinacia oleracea
2.4.1.14 ATP
-
Spinacia oleracea
2.4.1.14 fructose-1,6-bisphosphate
-
Spinacia oleracea
2.4.1.14 phosphate
-
Spinacia oleracea
2.4.1.14 UDP
-
Spinacia oleracea
2.4.1.14 UDP-glucose
-
Spinacia oleracea
2.4.1.14 UTP
-
Spinacia oleracea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.14 0.8
-
D-fructose 6-phosphate
-
Spinacia oleracea
2.4.1.14 7.1
-
UDP-glucose
-
Spinacia oleracea

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.14 Mg2+ no effect Spinacia oleracea

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.14 270000 280000 gel filtration Spinacia oleracea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate Spinacia oleracea catalyzes the penultimate step of sucrose synthesis UDP + sucrose 6-phosphate
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.14 Spinacia oleracea
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.14 partial Spinacia oleracea

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.14 leaf
-
Spinacia oleracea
-

Storage Stability

EC Number Storage Stability Organism
2.4.1.14 4°C, stable for at least 1 week Spinacia oleracea

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate ordered mechanism, highly specific for its substrates Spinacia oleracea UDP + sucrose 6-phosphate
-
?
2.4.1.14 UDP-alpha-D-glucose + D-fructose 6-phosphate catalyzes the penultimate step of sucrose synthesis Spinacia oleracea UDP + sucrose 6-phosphate
-
?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.14 6.9
-
-
Spinacia oleracea

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.14 6 8
-
Spinacia oleracea

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.1.14 0.7
-
UDP
-
Spinacia oleracea
2.4.1.14 0.8
-
fructose-1,6-bisphosphate
-
Spinacia oleracea
2.4.1.14 9.4
-
UDP-glucose
-
Spinacia oleracea
2.4.1.14 11
-
phosphate
-
Spinacia oleracea