EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.3.1.135 | 0.00046 | - |
all-trans-retinol | with dipalmitoylphosphatidylcholine, chemically dimerized enzyme | Bos taurus | |
2.3.1.135 | 0.00052 | - |
all-trans-retinol | with dipalmitoylphosphatidylcholine | Bos taurus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + all-trans-retinol | Bos taurus | - |
all-trans-retinyl acyl esters + 2-acylglycerophosphocholine | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.135 | Bos taurus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.3.1.135 | solubilization, centrifugation and dialysis | Bos taurus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.135 | phosphatidylcholine + all-trans-retinol | - |
Bos taurus | all-trans-retinyl acyl esters + 2-acylglycerophosphocholine | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.135 | homodimer | SDS-PAGE in absence of 2-mercaptoethanol | Bos taurus |
2.3.1.135 | homodimer | 2 × 25300, SDS-PAGE in presence of 2-mercaptoethanol | Bos taurus |
2.3.1.135 | monomer | - |
Bos taurus |
2.3.1.135 | More | LRAT monomer interact in membranes and form functional homodimers, the dimer formation is mediated by disulfide bond formation and protein-protein interactions | Bos taurus |