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Literature summary extracted from

  • Mondal, M.S.; Ruiz, A.; Hu, J.; Bok, D.; Rando, R.R.
    Two histidine residues are essential for catalysis by lecithin retinol acyl transferase (2001), FEBS Lett., 489, 14-18.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.135 expression in HEK-293T cells Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.135 0.0001
-
 all-trans-retinol with dipalmitoylphosphatidylcholine, H46Q mutant Homo sapiens
2.3.1.135 0.000167
-
 all-trans-retinol H126Q mutant Homo sapiens
2.3.1.135 0.00036
-
 all-trans-retinol with dipalmitoylphosphatidylcholine, cloned enzyme, expressed in HEK-293T cells Homo sapiens
2.3.1.135 0.00108
-
 all-trans-retinol with dipalmitoylphosphatidylcholine, H60Q mutant Homo sapiens
2.3.1.135 0.0024
-
 all-trans-retinol with dipalmitoylphosphatidylcholine, H72Q mutant Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.135 Bos taurus
-
-
-
2.3.1.135 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.135 enzyme transfected in HEK-293T cells partially purificated by solubilization and centrifugation Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.3.1.135 -70°C, 100 mM Tris-HCl, pH 8.3, 0.4% Triton X-100, stable for several days Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.135 dipalmitoylphosphatidylcholine + all-trans-retinol
-
 Bos taurus all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 ?
2.3.1.135 dipalmitoylphosphatidylcholine + all-trans-retinol mechanistic hypothesis Homo sapiens all-trans-retinyl palmitate + 2-palmitoylglycerophosphocholine
-
 ?