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Literature summary extracted from

  • Liu, J.J.; Odegard, W.; de Lumen, B.O.
    Galactinol synthase from kidney bean cotyledon and zucchini leaf. Purification and N-terminal sequences (1995), Plant Physiol., 109, 505-511.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.123 dithiothreitol absolute requirement Phaseolus vulgaris

General Stability

EC Number General Stability Organism
2.4.1.123 ammonium sulfate fractionation causes 32% loss of enzyme activity Phaseolus vulgaris
2.4.1.123 bovine serum albumin is essential to preserve enzyme activity during the assay Phaseolus vulgaris

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.123 0.4
-
 UDP-galactose
-
 Phaseolus vulgaris
2.4.1.123 4.5
-
 myo-inositol
-
 Phaseolus vulgaris

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.123 Mn2+ absolute requirement Phaseolus vulgaris

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.123 36000
-
 x * 36000, SDS-PAGE Cucurbita pepo
2.4.1.123 38000
-
 x * 38000, SDS-PAGE Phaseolus vulgaris

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol Cucurbita pepo
-
 UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?
2.4.1.123 UDP-galactose + myo-inositol Phaseolus vulgaris key enzyme in controlling galactose oligosaccharide biosynthesis, which plays a role in seed desiccation tolerance UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.123 Cucurbita pepo
-
 var. Black Beauty
-
2.4.1.123 Phaseolus vulgaris
-
 var. Big Red
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.123 1591fold, copurified with a 41 and 43 kDa peptide Phaseolus vulgaris
2.4.1.123 2472fold Cucurbita pepo

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.123 cotyledon highest activity among all of the components of seed, much poorer source than Cucurbita pepo mature leaves Phaseolus vulgaris
-
2.4.1.123 leaf mature leaves, much richer source than Phaseolus vulgaris cotyledons Cucurbita pepo
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.123 8.75
-
-
 Phaseolus vulgaris
2.4.1.123 32.1
-
-
 Cucurbita pepo

Storage Stability

EC Number Storage Stability Organism
2.4.1.123 -20°C, enzyme in ammonium sulfate precipitate form, 6-8 weeks, stable Phaseolus vulgaris

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.123 UDP-galactose + myo-inositol
-
 Cucurbita pepo UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?
2.4.1.123 UDP-galactose + myo-inositol
-
 Cucurbita pepo UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol ?
2.4.1.123 UDP-galactose + myo-inositol high specific affinity for UDP-Gal and myo-inositol Phaseolus vulgaris UDP + 1-O-alpha-D-galactosyl-D-myo-inositol identical with galactinol ?
2.4.1.123 UDP-galactose + myo-inositol key enzyme in controlling galactose oligosaccharide biosynthesis, which plays a role in seed desiccation tolerance Phaseolus vulgaris UDP + 1-O-alpha-D-galactosyl-D-myo-inositol
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.1.123 ? x * 38000, SDS-PAGE Phaseolus vulgaris
2.4.1.123 ? x * 36000, SDS-PAGE Cucurbita pepo

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.123 30
-
 assay at Phaseolus vulgaris
2.4.1.123 30
-
 assay at Cucurbita pepo

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.123 7
-
-
 Phaseolus vulgaris