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Literature summary extracted from

  • Kawashima, H.; Yamamoto, K.; Osawa, T.; Irimura, T.
    Purification and characterization of UDP-GlcNAc:Galbeta 1-4Glc(NAc)beta-1,3-N-acetylglucosaminyltransferase (poly-N-acetyllactosamine extension enzyme) from calf serum (1993), J. Biol. Chem., 268, 27118-27126.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.149 2-mercaptoethanol
-
Bos taurus
2.4.1.149 4-Thiouridine diphosphate inhibits in a concentration-dependent manner Bos taurus
2.4.1.149 CH3CN
-
Bos taurus
2.4.1.149 N-acetyllactosamine strong product inhibition Bos taurus
2.4.1.149 SDS
-
Bos taurus
2.4.1.149 UDP 1 mM, 70% inhibition Bos taurus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.149 0.129
-
UDP-N-acetyl-D-glucosamine
-
Bos taurus
2.4.1.149 18.2
-
lactose
-
Bos taurus
2.4.1.149 19.6
-
N-acetyllactosamine
-
Bos taurus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.149 Co2+ slightly stimulates, 12.8% as effective as Mn2+ at 10 mM Bos taurus
2.4.1.149 Mn2+ strict requirement Bos taurus
2.4.1.149 Mn2+ optimum concentration: 2-15 mM, cooperative activating effects by combination of Mn2+ at a low concentration and Mg2+ or Ca2+ Bos taurus
2.4.1.149 additional information enzyme possesses 2 distinct metal binding sites, not activated by Ca2+, Mg2+, Zn2+, Fe2+, Cu2+, Ni2+, K+, Na+ Bos taurus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.149 70000
-
1 * 70000, SDS-PAGE Bos taurus
2.4.1.149 90000
-
gel filtration Bos taurus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.149 UDP-N-acetyl-D-glucosamine + glycolipid Bos taurus physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + keratan sulfate Bos taurus physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + N-glycan Bos taurus physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + O-glycan Bos taurus physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine Bos taurus involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme ?
-
?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.149 Bos taurus
-
newborn calf
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.4.1.149 glycoprotein
-
Bos taurus

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.149 125000fold Bos taurus

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.149 serum newborn calf serum Bos taurus
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.149 0.118
-
-
Bos taurus

Storage Stability

EC Number Storage Stability Organism
2.4.1.149 4°C, purified enzyme, at least 2 weeks, stable Bos taurus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.149 additional information terminal Galbeta(1-4)Glc(NAc) sequences, i.e. type II chains, are preferred substrates, no substrates: terminal Galbeta(1-3)GlcNAc sequences, i.e. type I chains, Lewis X trisaccharides, i.e. Galbeta(1-4)(Fucalpha(1-3))GlcNAc, monosaccharides, e.g. galactose, asialo-bovine submaxillary mucin that contains GalNAcalpha1-Ser/Thr and Galbeta(1-3)GalNAcalpha1-Ser/Thr Bos taurus ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc i.e. lacto-N-neotetraose, 117% of activity with N-acetyllactosamine Bos taurus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + glycolipid physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates Bos taurus ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + keratan sulfate physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates Bos taurus ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + N-glycan physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates Bos taurus ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + O-glycan physiological acceptors: N-glycans, O-glycans, glycolipids and keratan sulfates Bos taurus ?
-
?
2.4.1.149 UDP-N-acetyl-D-glucosamine + poly-N-acetyllactosamine involved in the initiation and extension of poly-N-acetyllactosamine biosynthesis, may act as a key enzyme Bos taurus ?
-
?
2.4.1.149 UDP-N-acetylglucosamine + asialo-alpha1-acid glycoprotein best acceptor among the glycoproteins that contain Galbeta(1-4)GlcNAc Bos taurus UDP + N-acetylglucosaminylated asialo-alpha1-acid glycoprotein
-
?
2.4.1.149 UDP-N-acetylglucosamine + lactose transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage, 51% of activity with N-acetyllactosamine Bos taurus UDP + GlcNAcbeta(1-3)Galbeta(1-4)Glc
-
?
2.4.1.149 UDP-N-acetylglucosamine + N-acetyllactosamine transfer of one GlcNAc to position C-3 of the terminal Gal residue of lactose or N-acetyllactosamine in the beta linkage Bos taurus UDP + GlcNAcbeta(1-3)Galbeta(1-4)GlcNAc
-
?

Subunits

EC Number Subunits Comment Organism
2.4.1.149 monomer 1 * 70000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.149 37
-
assay at Bos taurus
2.4.1.149 42
-
112% of activity compared with that at 37°C Bos taurus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.149 4
-
4% of activity compared with that at 37°C Bos taurus
2.4.1.149 12
-
9% of activity compared with that at 37°C Bos taurus
2.4.1.149 20
-
28% of activity compared with that at 37°C Bos taurus
2.4.1.149 30
-
63% of activity compared with that at 37°C Bos taurus
2.4.1.149 42
-
112% of activity compared with that at 37°C Bos taurus
2.4.1.149 45
-
88% of activity compared with that at 37°C Bos taurus
2.4.1.149 50
-
10% of activity compared with that at 37°C Bos taurus
2.4.1.149 55
-
2% of activity compared with that at 37°C Bos taurus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.149 7
-
broad pH-optimum around pH 7 Bos taurus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.149 6 7.5 substantial reaction in the range, 65% of activity at pH 7 Bos taurus