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Literature summary extracted from

  • Holmes, E.H.
    Characterization of a beta 1-->3-N-acetylglucosaminyltransferase associated with synthesis of type 1 and type 2 lacto-series tumor-associated antigens from the human colonic adenocarcinoma cell line SW403 (1988), Arch. Biochem. Biophys., 260, 461-468.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.149 CHAPSO activates solubilized enzyme Homo sapiens
2.4.1.149 deoxycholate activates solubilized enzyme Homo sapiens
2.4.1.149 G-3634-A activates solubilized enzyme Homo sapiens
2.4.1.149 additional information CDPcholine stimulates by protecting UDP-GlcNAc from hydrolysis by endogenous enzymes Homo sapiens
2.4.1.149 Triton CF-54 activates solubilized enzyme, activity is optimal when assayed in the presence of a final concentration of 0.3% Homo sapiens
2.4.1.149 Triton X-100 activates solubilized enzyme Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.149 EDTA 10 mM, complete inhibition Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.149 0.17
-
 UDP-N-acetyl-D-glucosamine lactosylceramide as acceptor Homo sapiens
2.4.1.149 0.19
-
 lactoneotetraosylceramide
-
 Homo sapiens
2.4.1.149 0.19
-
 lactonorhexaosylceramide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.149 membrane membrane-bound Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.149 Mn2+ strict requirement Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.149 additional information Homo sapiens biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.149 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.149 additional information enzyme is undetectable in normal colonic epithelial cells, but is greatly enhanced both during fetal development and in colonic adenocarcinomas Homo sapiens
-
2.4.1.149 SW-403 cell colonic adenocarcinoma cell line Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.149 additional information transfer of GlcNAc occurs mainly to type 2 chain nonfucosylated structures, elongation of type 1 chain structure Lc4 is also detectable, no transfer to any fucosylated derivative of either type 1 or 2 chains, transfer of GlcNAc to a terminal Gal residue on a lacto-series core chain Homo sapiens ?
-
 ?
2.4.1.149 additional information biosynthesis of lacto-series core chains, enzyme is activated in association with oncogenesis in colonic epithelia Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + lactoneotetraosylceramide lactoneotetraosylceramide from bovine erythrocytes, higher rate than with lactonorhexaosylceramide, efficiency decreases with growing acceptor chain length Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + lactonorhexaosylceramide lactonorhexaosylceramide from bovine erythrocytes, lower rate than with lactoneotetraosylceramide, efficiency decreases with growing acceptor chain length Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + lactosylceramide lactosylceramide from human erythrocytes Homo sapiens ?
-
 ?
2.4.1.149 UDP-N-acetylglucosamine + lactotetraosylceramide lactotetraosylceramide from human meconium, poor substrate Homo sapiens ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.149 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.149 5.8 7.5 broad Homo sapiens
2.4.1.149 7 7.2 highest activity, with Hepes buffer Homo sapiens