EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.131 | ADP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | ATP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | deoxycholate | - |
Oryctolagus cuniculus | |
2.4.1.131 | GDP | 0.02 mM, 84% inhibition; 2 mM, 84% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | GMP | 0.02 mM, 30% inhibition; 2 mM, 30% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | GTP | 0.02 mM, 40% inhibition; 2 mM, 40% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | additional information | less than 15% inhibition at 2 mM: ADP, ATP, UDP, UDP-glucose, UDP-galactose and UDP-N-acetylglucosamine; not inhibited by EDTA | Oryctolagus cuniculus | |
2.4.1.131 | taurocholate | - |
Oryctolagus cuniculus | |
2.4.1.131 | UDP | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | UDP-galactose | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | UDP-glucose | 2 mM, less than 15% inhibition | Oryctolagus cuniculus | |
2.4.1.131 | UDP-N-acetylglucosamine | 2 mM, less than 15% inhibition | Oryctolagus cuniculus |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.1.131 | 0.00064 | - |
D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | pH 7.0, 37°C | Oryctolagus cuniculus | |
2.4.1.131 | 0.00064 | - |
oligosaccharide-lipid acceptor | based on the assumption of incorporation of only a single mannosyl unit into the product | Oryctolagus cuniculus | |
2.4.1.131 | 0.0047 | - |
GDPmannose | - |
Oryctolagus cuniculus | |
2.4.1.131 | 0.0047 | - |
GDP-D-mannose | pH 7.0, 37°C | Oryctolagus cuniculus |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.4.1.131 | microsome | - |
Oryctolagus cuniculus | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.4.1.131 | additional information | no requirement of divalent cations | Oryctolagus cuniculus | |
2.4.1.131 | additional information | reaction is independent of the addition of divalent cations | Oryctolagus cuniculus |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.131 | GDPmannose + oligosaccharide-lipid acceptor | Oryctolagus cuniculus | biosynthesis of oligosaccharide-lipids | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.1.131 | Oryctolagus cuniculus | - |
- |
- |
2.4.1.131 | Oryctolagus cuniculus | - |
New Zealand white rabbits | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.1.131 | partial | Oryctolagus cuniculus |
2.4.1.131 | solubilized with 0.15% v/v Nonidet P-40, partial | Oryctolagus cuniculus |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.4.1.131 | liver | - |
Oryctolagus cuniculus | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.4.1.131 | additional information | - |
- |
Oryctolagus cuniculus |
EC Number | Storage Stability | Organism |
---|---|---|
2.4.1.131 | 0-4°C, solubilized enzyme, at least 2 weeks, stable | Oryctolagus cuniculus |
2.4.1.131 | 0°C, partially purified enzyme, 5 days, stable | Oryctolagus cuniculus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.1.131 | 2 GDP-alpha-D-mannose + D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | - |
Oryctolagus cuniculus | 2 UDP + D-Man-alpha-(1->2)-D-Man-alpha-(1->2)-D-Man-alpha-(1->3)-(D-Man-alpha-(1->6))-D-Man-beta-(1->4)-D-GlcNAc-beta-(1->4)-D-GlcNAc-diphosphodolichol | - |
? | |
2.4.1.131 | GDP-mannose + oligosaccharide-lipid acceptor | acceptor isolated from pig liver | Oryctolagus cuniculus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | presumably a heptasaccharide with a 1,6-linked branched glycosyl unit with 65% of the newly added mannosyl units in a terminal nonreducing position, structure may be Manalpha(1-2)Man-Man(Man(1-6))Man-GlcNAc-GlcNAc | ? | |
2.4.1.131 | GDPmannose + oligosaccharide-lipid acceptor | biosynthesis of oligosaccharide-lipids | Oryctolagus cuniculus | GDP + alpha-mannosyl-1,2-oligosaccharide-lipid | - |
? | |
2.4.1.131 | additional information | no substrate: dolichol-phosphat | Oryctolagus cuniculus | additional information | - |
? |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.4.1.131 | 37 | - |
assay at | Oryctolagus cuniculus |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.1.131 | 6.5 | 7.5 | - |
Oryctolagus cuniculus |