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Literature summary extracted from

  • Leer, J.C.; Hammer-Jespersen, K.; Schwartz, M.
    Uridine phosphorylase from Escherichia coli. Physical and chemical characterization (1977), Eur. J. Biochem., 75, 217-224.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 additional information
-
 additional information
-
 Escherichia coli

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 22200
-
 8 * 22200, SDS-PAGE Escherichia coli
2.4.2.3 160000
-
 gel filtration Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3 homogeneity Escherichia coli

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 133
-
-
 Escherichia coli

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 2'-deoxyuridine + phosphate 6% of the activity with uridine Escherichia coli uracil + deoxyribose 1-phosphate
-
 ?
2.4.2.3 5-bromo-2'-deoxyuridine + phosphate 27% of the activity with uridine Escherichia coli 5-bromouracil + 2-deoxribose 1-phosphate
-
 ?
2.4.2.3 5-bromouridine + phosphate 69% of the activity with uridine Escherichia coli 5-bromouracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 5-fluoro-2'-deoxyuridine + phosphate 14% of the activity with uridine Escherichia coli 5-fluorouracil + 2-deoxyribose-1-phosphate
-
 ?
2.4.2.3 5-methyluridine + phosphate 19% of the activity with uridine Escherichia coli thymine + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 thymidine + phosphate 2% of the activity with uridine Escherichia coli thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Escherichia coli uracil + alpha-D-ribose 1-phosphate
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.2.3 octamer 8 * 22200, SDS-PAGE Escherichia coli

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.3 37
-
 assay at Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7.3
-
 uridine phosphorolysis Escherichia coli

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.3 5.8 9 pH 5.8: about 50% of activity maximum, pH 9.0: about 40% of activity maximum Escherichia coli