Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Yamada, E.W.
    Uridine phosphorylase from rat liver (1978), Methods Enzymol., 51, 423-431.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 5,5'-dithiobis(2-nitrobenzoic acid)
-
 Rattus norvegicus
2.4.2.3 Deoxyglucosylthymine phosphorolysis of uridine and deoxyuridine, synthesis of uridine at concentrations of 0.10 mM, 0.018 mM and 0.14 mM, not: phosphorolysis of deoxyuridine or thymidine at 0.19 mM Rattus norvegicus
2.4.2.3 iodoacetamide
-
 Rattus norvegicus
2.4.2.3 iodoacetic acid
-
 Rattus norvegicus
2.4.2.3 N-ethylmaleimide
-
 Rattus norvegicus
2.4.2.3 o-Iodosobenzoate
-
 Rattus norvegicus
2.4.2.3 p-chloromercuribenzoate
-
 Rattus norvegicus
2.4.2.3 p-Mercuriphenylsulfonate
-
 Rattus norvegicus
2.4.2.3 Uracil
-
 Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.3 cytosol
-
 Rattus norvegicus 5829
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 26000
-
 4 * 26000, SDS-PAGE in presence of 4 M urea and 0.5% 2-mercaptoethanol Rattus norvegicus
2.4.2.3 110000
-
 gel filtration Rattus norvegicus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.3 uridine + phosphate Rattus norvegicus role in degradation of pyrimidine nucleosides as well as in the salvage pathway for nucleic acid synthesis uracil + alpha-D-ribose 1-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Rattus norvegicus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.3 uridine + phosphate = uracil + alpha-D-ribose 1-phosphate ordered bi-bi mechanism Rattus norvegicus
a
2.4.2.3 uridine + phosphate = uracil + alpha-D-ribose 1-phosphate phosphate binds before uridine and ribose 1-phosphate is released after uracil Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 2.4
-
-
 Rattus norvegicus

Storage Stability

EC Number Storage Stability Organism
2.4.2.3 -40°C, 0.05 M potassium phosphate buffer, pH 7.0, 10 mM 2-mercaptoethanol, 1 mM EDTA, 10% loss of activity after 6 weeks Rattus norvegicus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 uridine + phosphate
-
 Rattus norvegicus uracil + alpha-D-ribose 1-phosphate
-
 r
2.4.2.3 uridine + phosphate role in degradation of pyrimidine nucleosides as well as in the salvage pathway for nucleic acid synthesis Rattus norvegicus uracil + alpha-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 tetramer 4 * 26000, SDS-PAGE in presence of 4 M urea and 0.5% 2-mercaptoethanol Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.3 37
-
 reverse reaction, assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 6.5
-
 deoxyuridine phosphorolysis Rattus norvegicus
2.4.2.3 8.2
-
 reverse assay, uridine phosphorolysis Rattus norvegicus
2.4.2.3 8.5
-
 uridine synthesis Rattus norvegicus