Literature summary extracted from

Smith, C.V.; Huang, C.C.; Miczak, A.; Russell, D.G.; Sacchettini, J.C.; Honer zu Bentrup, K.
Biochemical and structural studies of malate synthase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 1735-1743.

Cloned(Commentary)

EC Number	Cloned (Comment)	Organism
2.3.3.9	-	Mycobacterium tuberculosis

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.3.3.9	mixed alpha/beta structure	Mycobacterium tuberculosis

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.3.9	Bromopyruvate	-	Mycobacterium tuberculosis
2.3.3.9	glycolate	only at fairly high concentration	Mycobacterium tuberculosis
2.3.3.9	malate	1.0 mM, 50% inhibition	Mycobacterium tuberculosis
2.3.3.9	additional information	3-phosphoglycerate, 6-phosphogluconate, malonic acid; not: fructose 1,6-bisphosphate	Mycobacterium tuberculosis
2.3.3.9	oxalate	-	Mycobacterium tuberculosis
2.3.3.9	phosphoenolpyruvate	-	Mycobacterium tuberculosis

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3.3.9	0.03	-	acetyl-CoA	recombinant enzyme	Mycobacterium tuberculosis
2.3.3.9	0.057	-	glyoxylate	recombinant enzyme	Mycobacterium tuberculosis

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.3.9	Mg2+	absolute requirement for divalent cation, maximal activity with 5 mM Mg2+	Mycobacterium tuberculosis
2.3.3.9	Mn2+	40% of the activation with Mg2+	Mycobacterium tuberculosis
2.3.3.9	additional information	Co2+, Fe2+, Ca2+, Ba2+, Ni2+, Cd2+, Zn2+, Cu2+, Hg2+ are not able to support the activity of enzyme	Mycobacterium tuberculosis

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.3.9	Mycobacterium tuberculosis	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.3.9	recombinant enzyme	Mycobacterium tuberculosis

Reaction

EC Number	Reaction	Comment	Organism	Reaction ID
2.3.3.9	acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA	mechanism	Mycobacterium tuberculosis	a

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.3.9	6	-	recombinant enzyme	Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.3.9	glyoxylate + acetyl-CoA + H2O	structure of enzyme-substrate complex	Mycobacterium tuberculosis	(S)-malate + CoA	-	?

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.3.9	7.5	-	-	Mycobacterium tuberculosis

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.3.3.9	0.06	-	Bromopyruvate	-	Mycobacterium tuberculosis
2.3.3.9	0.2	-	phosphoenolpyruvate	-	Mycobacterium tuberculosis
2.3.3.9	0.4	-	oxalate	-	Mycobacterium tuberculosis
2.3.3.9	0.9	-	glycolate	-	Mycobacterium tuberculosis

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Release 2023.1 (January 2023)