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Literature summary extracted from

  • Reinscheid, D.J.; Eikmanns, B.J.; Sahm, H.
    Malate synthase from Corynebacterium glutamicum: sequence analysis of the gene and biochemical characterization of the enzyme (1994), Microbiology, 140, 3099-3108.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.3.9 expression in Escherichia coli and Corynebacterium glutamicum Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 ATP
-
 Corynebacterium glutamicum
2.3.3.9 glycolate
-
 Corynebacterium glutamicum
2.3.3.9 additional information acetyl-phosphate; not: fructose 1,6-bisphosphate Corynebacterium glutamicum
2.3.3.9 oxalate
-
 Corynebacterium glutamicum
2.3.3.9 phosphoenolpyruvate not Corynebacterium glutamicum
2.3.3.9 pyruvate not Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.012
-
 acetyl-CoA
-
 Corynebacterium glutamicum
2.3.3.9 0.03
-
 glyoxylate
-
 Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Co2+ 25% of the activation with Mg2+ Corynebacterium glutamicum
2.3.3.9 Mg2+ absolute requirement for divalent cation, maximal activity with 40 mM Mg2+ Corynebacterium glutamicum
2.3.3.9 Mn2+ 15% of the activation with Mg2+ Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 80000
-
 gel filtration Corynebacterium glutamicum
2.3.3.9 90000
-
 1 * 90000, SDS-PAGE Corynebacterium glutamicum

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Corynebacterium glutamicum P42450
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Corynebacterium glutamicum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 28.6
-
-
 Corynebacterium glutamicum

Storage Stability

EC Number Storage Stability Organism
2.3.3.9 4°C, stable for more than 3 weeks Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Corynebacterium glutamicum (S)-malate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.3.3.9 monomer 1 * 90000, SDS-PAGE Corynebacterium glutamicum
2.3.3.9 monomer 1* 82362, calculation from nucleotide sequence Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.9 43
-
-
 Corynebacterium glutamicum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.3.9 0.27
-
 oxalate
-
 Corynebacterium glutamicum
2.3.3.9 0.44
-
 glycolate
-
 Corynebacterium glutamicum
2.3.3.9 4.3
-
 ATP
-
 Corynebacterium glutamicum