Literature summary extracted from

Dixon, G.H.; Kornberg, H.L.; Lund, P.
Purification and properties of malate synthetase (1960), Biochim. Biophys. Acta, 41, 217-233.

General Stability

EC Number	General Stability	Organism
2.3.3.9	freezing and thawing inactivates	Pseudomonas putida
2.3.3.9	Mg2+ stabilizes	Saccharomyces cerevisiae
2.3.3.9	Mg2+ stabilizes	Pseudomonas putida

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
2.3.3.9	fluoroacetate	-	Pseudomonas putida
2.3.3.9	fluoroacetate	-	Saccharomyces cerevisiae
2.3.3.9	glycolate	-	Pseudomonas putida
2.3.3.9	glycolate	-	Saccharomyces cerevisiae
2.3.3.9	oxalate	-	Pseudomonas putida
2.3.3.9	oxalate	-	Saccharomyces cerevisiae

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
2.3.3.9	0.063	-	glyoxylate	-	Pseudomonas putida
2.3.3.9	0.093	-	glyoxylate	-	Saccharomyces cerevisiae

Metals/Ions

EC Number	Metals/Ions	Comment	Organism	Structure
2.3.3.9	Mg2+	required	Saccharomyces cerevisiae
2.3.3.9	Mg2+	required	Pseudomonas putida
2.3.3.9	Mg2+	Km: 0.5 mM	Saccharomyces cerevisiae
2.3.3.9	Mg2+	Km: 0.5 mM	Pseudomonas putida
2.3.3.9	Mn2+	can partially replace Mg2+ in activation	Saccharomyces cerevisiae
2.3.3.9	Mn2+	can partially replace Mg2+ in activation	Pseudomonas putida

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.3.3.9	Pseudomonas putida	-	-	-
2.3.3.9	Saccharomyces cerevisiae	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
2.3.3.9	-	Saccharomyces cerevisiae
2.3.3.9	-	Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
2.3.3.9	additional information	-	-	Saccharomyces cerevisiae
2.3.3.9	additional information	-	-	Pseudomonas putida

Storage Stability

EC Number	Storage Stability	Organism
2.3.3.9	2°C, stable for at least a month	Saccharomyces cerevisiae
2.3.3.9	2°C, stable for at least a month	Pseudomonas putida

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.3.3.9	glyoxylate + acetyl-CoA + H2O	-	Saccharomyces cerevisiae	(S)-malate + CoA	-	ir
2.3.3.9	glyoxylate + acetyl-CoA + H2O	-	Pseudomonas putida	(S)-malate + CoA	-	ir
2.3.3.9	glyoxylate + fluoroacetyl-CoA + H2O	-	Pseudomonas putida	2-oxo-3-fluoro-butanedioic acid + CoA	-	ir

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.3.3.9	additional information	-	additional information	-	Saccharomyces cerevisiae
2.3.3.9	additional information	-	additional information	-	Pseudomonas putida

pH Optimum

EC Number	pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
2.3.3.9	8.5	-	-	Saccharomyces cerevisiae
2.3.3.9	8.5	-	-	Pseudomonas putida

Ki Value [mM]

EC Number	Ki Value [mM]	Ki Value maximum [mM]	Inhibitor	Comment	Organism	Structure
2.3.3.9	0.019	-	oxalate	-	Saccharomyces cerevisiae
2.3.3.9	0.019	-	oxalate	-	Pseudomonas putida
2.3.3.9	0.246	-	fluoroacetate	-	Saccharomyces cerevisiae
2.3.3.9	0.246	-	fluoroacetate	-	Pseudomonas putida
2.3.3.9	0.308	-	glycolate	-	Saccharomyces cerevisiae
2.3.3.9	0.308	-	glycolate	-	Pseudomonas putida

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Release 2023.1 (January 2023)