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Literature summary extracted from

  • Miernyk, J.A.; Trelease, R.N.
    Malate synthase from Gossypium hirsutum (1981), Phytochemistry, 20, 2657-2663.
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.3.9 Triton X-100 activation Gossypium hirsutum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.3.9 acetaldehyde weak Gossypium hirsutum
2.3.3.9 ADP
-
 Gossypium hirsutum
2.3.3.9 ATP
-
 Gossypium hirsutum
2.3.3.9 Butanedione slight inhibition at high concentrations Gossypium hirsutum
2.3.3.9 chloroacetyl-CoA
-
 Gossypium hirsutum
2.3.3.9 deamino-acetyl-CoA
-
 Gossypium hirsutum
2.3.3.9 deoxycholate
-
 Gossypium hirsutum
2.3.3.9 dephosphoacetyl-CoA
-
 Gossypium hirsutum
2.3.3.9 diethyl dicarbonate inhibition prevented by preincubation with acetyl-CoA Gossypium hirsutum
2.3.3.9 glycolate
-
 Gossypium hirsutum
2.3.3.9 methylglyoxal
-
 Gossypium hirsutum
2.3.3.9 oxalate
-
 Gossypium hirsutum
2.3.3.9 oxamate
-
 Gossypium hirsutum
2.3.3.9 pyridoxal-5'-phosphate preincubation with glyoxylate but not acetyl-CoA prevents inhibition Gossypium hirsutum
2.3.3.9 pyruvate weak Gossypium hirsutum
2.3.3.9 S-acetonyl-CoA
-
 Gossypium hirsutum
2.3.3.9 S-ethyl-CoA
-
 Gossypium hirsutum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.3.9 0.01
-
 acetyl-CoA
-
 Gossypium hirsutum
2.3.3.9 0.052
-
 glyoxylate
-
 Gossypium hirsutum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.3.9 Ba2+ about 30% of the activation with Mg2+ Gossypium hirsutum
2.3.3.9 Co2+ about 30% of the activation with Mg2+ Gossypium hirsutum
2.3.3.9 Mg2+ Km: 0.59 mM Gossypium hirsutum
2.3.3.9 Mg2+ absolute requirement for divalent metal ion, best fulfilled by Mg2+ Gossypium hirsutum
2.3.3.9 Mn2+ about 30% of the activation with Mg2+ Gossypium hirsutum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.3.3.9 730000
-
 gel filtration Gossypium hirsutum
2.3.3.9 750000
-
 sucrose density gradient centrifugation Gossypium hirsutum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O Gossypium hirsutum enzyme specifically involved in glyoxalate cycle metabolism (S)-malate + CoA
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.3.9 Gossypium hirsutum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.3.9
-
 Gossypium hirsutum

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.3.9 acetyl-CoA + glyoxylate + H2O = (S)-malate + CoA sequential bireactant mechanism Gossypium hirsutum
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.3.9 cotyledon of dark-germinated seeds Gossypium hirsutum
-
2.3.3.9 seed
-
 Gossypium hirsutum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.3.3.9 additional information
-
-
 Gossypium hirsutum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.3.9 glyoxylate + acetyl-CoA + H2O
-
 Gossypium hirsutum (S)-malate + CoA
-
 ir
2.3.3.9 glyoxylate + acetyl-CoA + H2O enzyme specifically involved in glyoxalate cycle metabolism Gossypium hirsutum (S)-malate + CoA
-
 ?
2.3.3.9 glyoxylate + butyryl-CoA + H2O
-
 Gossypium hirsutum 2-oxohexanedioic acid + CoA
-
 ir
2.3.3.9 glyoxylate + propionyl-CoA + H2O
-
 Gossypium hirsutum 2-oxopentanedioic acid + CoA
-
 ir

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.3.9 40
-
-
 Gossypium hirsutum

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.3.9 65
-
 2 min, 84% loss of activity Gossypium hirsutum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.3.9 8
-
 Tris/HCl buffer Gossypium hirsutum
2.3.3.9 8.2
-
 phosphate-citrate-borate buffer or MOPS-KOH buffer Gossypium hirsutum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.3.3.9 6.8 9.7 pH 6.8 and 9.7: about 50% of maximal activity, phosphate-citrate-borate buffer Gossypium hirsutum