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Literature summary extracted from

  • Avraham, Y.; Grossowicz, N.; Yashphe, J.
    Purification and characterization of uridine and thymidine phosphorylase from Lactobacillus casei (1990), Biochim. Biophys. Acta, 1040, 287-293.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.3 3-O-methyl-alpha-D-glucopyranose
-
 Lacticaseibacillus casei
2.4.2.3 p-chloromercuribenzoate
-
 Lacticaseibacillus casei
2.4.2.4 2-deoxy-D-ribose competitive Lacticaseibacillus casei
2.4.2.4 deoxyribose-1-phosphate
-
 Lacticaseibacillus casei
2.4.2.4 thymine
-
 Lacticaseibacillus casei

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.3 additional information
-
 additional information
-
 Lacticaseibacillus casei
2.4.2.3 4
-
 5-methyluridine
-
 Lacticaseibacillus casei
2.4.2.4 additional information
-
 additional information
-
 Lacticaseibacillus casei
2.4.2.4 0.1
-
 phosphate
-
 Lacticaseibacillus casei

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.3 20000
-
 4 * 20000, SDS-PAGE in presence of 2-mercaptoethanol Lacticaseibacillus casei
2.4.2.3 80000
-
 non-denaturing PAGE Lacticaseibacillus casei
2.4.2.4 38000
-
 2 * 38000, SDS-PAGE Lacticaseibacillus casei
2.4.2.4 80000
-
 gel filtration Lacticaseibacillus casei

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.3 Lacticaseibacillus casei
-
-
-
2.4.2.4 Lacticaseibacillus casei
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.3
-
 Lacticaseibacillus casei
2.4.2.4 homogeneity Lacticaseibacillus casei

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.3 uridine + phosphate = uracil + alpha-D-ribose 1-phosphate sequential rather than ping-pong mechanism, addition of substrate is random Lacticaseibacillus casei
a
2.4.2.4 thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate mechanism Lacticaseibacillus casei
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.3 2810
-
-
 Lacticaseibacillus casei
2.4.2.4 1100
-
-
 Lacticaseibacillus casei

Storage Stability

EC Number Storage Stability Organism
2.4.2.3 5°C, 90% loss of activity after 3-4 days Lacticaseibacillus casei
2.4.2.4 5°C, 90% loss of activity after 3-4 days Lacticaseibacillus casei

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.3 5-methyluridine + phosphate
-
 Lacticaseibacillus casei 5-methyluracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 additional information no substrate: cytidine Lacticaseibacillus casei ?
-
 ?
2.4.2.3 additional information no substrates: 2-deoxyuridine, adenosine Lacticaseibacillus casei ?
-
 ?
2.4.2.3 thymidine + phosphate
-
 Lacticaseibacillus casei thymine + 2-deoxy-alpha-D-ribose 1-phosphate
-
 ?
2.4.2.3 uracil arabinoside + phosphate
-
 Lacticaseibacillus casei uracil + arabinose-1-phosphate
-
 ?
2.4.2.3 uridine + phosphate
-
 Lacticaseibacillus casei uracil + alpha-D-ribose 1-phosphate
-
 ?
2.4.2.4 5-deoxythymidine + phosphate
-
 Lacticaseibacillus casei thymine + 2,5-dideoxyribose 1-phosphate
-
 ?
2.4.2.4 5-fluorouridine + phosphate
-
 Lacticaseibacillus casei 5-fluorouracil + ribose 1-phosphate
-
 ?
2.4.2.4 bromodeoxyuridine + phosphate
-
 Lacticaseibacillus casei bromouracil + 2-deoxy-D-ribose
-
 ?
2.4.2.4 deoxyuridine + phosphate
-
 Lacticaseibacillus casei uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 additional information specificity towards the deoxyribosyl moiety of the substrate Lacticaseibacillus casei ?
-
 ?
2.4.2.4 additional information nonsubstituted pyrimidine moiety or one which is substituted in position 5 required Lacticaseibacillus casei ?
-
 ?
2.4.2.4 thymidine + phosphate
-
 Lacticaseibacillus casei thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 thymine arabinoside + phosphate
-
 Lacticaseibacillus casei thymine + arabinose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.3 tetramer 4 * 20000, SDS-PAGE in presence of 2-mercaptoethanol Lacticaseibacillus casei
2.4.2.4 dimer 2 * 38000, SDS-PAGE Lacticaseibacillus casei

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.3 7
-
-
 Lacticaseibacillus casei
2.4.2.4 6
-
-
 Lacticaseibacillus casei

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.4 0.67
-
 deoxyribose-1-phosphate
-
 Lacticaseibacillus casei