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Literature summary extracted from

  • Blank, J.G.; Hoffee, P.A.
    Purification and properties of thymidine phosphorylase from Salmonella typhimurium (1975), Arch. Biochem. Biophys., 168, 259-265.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.4 2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 quite labile during purification Salmonella enterica subsp. enterica serovar Typhimurium

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.4 2-deoxy-D-ribose product inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 5-Hydroxymethyluracil 35% inactivation at 2 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 Bromouracil 100% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 deoxyadenosine 20% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 ribose 1-phosphate 20% inactivation at 1.5 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 thymine product inhibition Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 Uracil 84% inactivation at 2 mM Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 uridine competitive 27% inactivation at 1 mM Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.4 1.3
-
 arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 2.1
-
 thymidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 2.3
-
 phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 8
-
 deoxyuridine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.4 47000
-
 2 * 47000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 100000
-
 gel filtration Salmonella enterica subsp. enterica serovar Typhimurium

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.4 Salmonella enterica subsp. enterica serovar Typhimurium
-
 LT-2
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.4 257fold Salmonella enterica subsp. enterica serovar Typhimurium

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.4 thymidine + phosphate = thymine + 2-deoxy-alpha-D-ribose 1-phosphate ordered bi bi mechanism with the nucleoside the first substrate to add, and the pyrimidine base the last product to leave Salmonella enterica subsp. enterica serovar Typhimurium
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.4 465
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.4.2.4 4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.4 bromodeoxyuridine + phosphate at 40% the rate of thymidine Salmonella enterica subsp. enterica serovar Typhimurium bromouracil + 2-deoxy-D-ribose
-
 ?
2.4.2.4 deoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 iododeoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium iodouracil + 2-deoxy-D-ribose
-
 ?
2.4.2.4 additional information no substrate: deoxycytidine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 additional information no substrates: deoxyadenosine, deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 additional information specific for deoxyribonucleosides Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 thymidine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 thymidine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.4 dimer 2 * 47000, SDS-disc gel electrophoresis Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.4 7.5 8 rapid decrease of activity above and below this range Salmonella enterica subsp. enterica serovar Typhimurium