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Literature summary extracted from

  • Hoffee, P.A.; Blank, J.
    Thymidine phosphorylase from Salmonella typhimurium (1978), Methods Enzymol., 51, 437-442.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.2.4 2-mercaptoethanol and sucrose stabilize during preincubation procedure with ammonium sulfate Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 quite labile during purification Salmonella enterica subsp. enterica serovar Typhimurium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.4 1.3
-
 arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 2.1
-
 thymidine
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 2.3
-
 phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 8
-
 deoxyuridine
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.4 47000
-
 2 * 47000 Salmonella enterica subsp. enterica serovar Typhimurium

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.4 thymine + 2-deoxy-D-ribose 1-phosphate Salmonella enterica subsp. enterica serovar Typhimurium in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine thymidine + phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.4 Salmonella enterica subsp. enterica serovar Typhimurium
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.4
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.4 465
-
-
 Salmonella enterica subsp. enterica serovar Typhimurium

Storage Stability

EC Number Storage Stability Organism
2.4.2.4 4°C, 10 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol, 20% sucrose, stable for 3 months Salmonella enterica subsp. enterica serovar Typhimurium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.4 bromodeoxyuridine + phosphate at 40% the rate of thymidine Salmonella enterica subsp. enterica serovar Typhimurium bromouracil + 2-deoxy-D-ribose
-
 ?
2.4.2.4 deoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium uracil + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 iododeoxyuridine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium iodouracil + 2-deoxy-D-ribose
-
 ?
2.4.2.4 additional information no substrate: deoxycytidine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 additional information no substrates: deoxyadenosine, deoxyguanosine Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 thymidine + arsenate
-
 Salmonella enterica subsp. enterica serovar Typhimurium ?
-
 ?
2.4.2.4 thymidine + phosphate
-
 Salmonella enterica subsp. enterica serovar Typhimurium thymine + 2-deoxy-D-ribose 1-phosphate
-
 ?
2.4.2.4 thymine + 2-deoxy-D-ribose 1-phosphate in Escherichia coli and Salmonella typhimurium thymidine phosphorylase plays an important role in metabolism of thymine auxotrophs and is necessary for the conversion of exogenous thymine to thymidine Salmonella enterica subsp. enterica serovar Typhimurium thymidine + phosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.4 dimer 2 * 47000 Salmonella enterica subsp. enterica serovar Typhimurium

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.4 37
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.4 7.4
-
 assay at Salmonella enterica subsp. enterica serovar Typhimurium
2.4.2.4 7.5 8 rapid decrease of activity above and below this range Salmonella enterica subsp. enterica serovar Typhimurium

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.4 7.5 8 rapid decrease of activity above and below this range Salmonella enterica subsp. enterica serovar Typhimurium