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Literature summary extracted from

  • Ihara, M.; Tanaka, Y.; Yanagisawa, K.; Taya, Y.; Nishimura, S.
    Purification and some properties of discadenine synthase from Dictyostelium discoideum (1986), Biochim. Biophys. Acta, 881, 135-140.
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.24 Ca2+
-
 Dictyostelium discoideum
2.5.1.24 Mg2+ 100 mM, 30% inhibition Dictyostelium discoideum
2.5.1.24 Mn2+
-
 Dictyostelium discoideum
2.5.1.24 Zn2+ 100 mM, 80% inhibition Dictyostelium discoideum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.24 0.0007
-
 N6-benzyladenine
-
 Dictyostelium discoideum
2.5.1.24 0.0185
-
 S-adenosyl-L-methionine
-
 Dictyostelium discoideum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.24 82000
-
 gel filtration Dictyostelium discoideum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.24 Dictyostelium discoideum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.24
-
 Dictyostelium discoideum

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.24 fruit body
-
 Dictyostelium discoideum
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.24 0.0123
-
-
 Dictyostelium discoideum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.24 S-adenosyl-L-methionine + N6-(DELTA2-isopentenyl)-adenine
-
 Dictyostelium discoideum 5'-methylthioadenosine + discadenine discadenine is 3-(3-amino-3-carboxypropyl)-N6-(DELTA2-isopentenyl)-adenine ?
2.5.1.24 S-adenosylmethionine + N6-benzyladenine
-
 Dictyostelium discoideum 3-(3-amino-3-carboxypropyl)-N6-benzyladenine + 5'-methylthioadenosine
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.5.1.24 35
-
-
 Dictyostelium discoideum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.24 7.5
-
-
 Dictyostelium discoideum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.24 6 8.5 pH 6.0: about 55% of activity maximum, pH 8.5: about 60% of activity maximum Dictyostelium discoideum