EC Number | Application | Comment | Organism |
---|---|---|---|
2.4.2.8 | medicine | potential target for antiparasitic chemotherapy | Leishmania tarentolae |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.4.2.8 | DNA and amino acid sequence determination and analysis, functional expression in Escherichia coli BL21(DE3) | Leishmania tarentolae |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.4.2.8 | recombinant enzyme, 7 mg/ml, hanging-drop vapour-diffusion method, TMD buffer, pH 7.5, + equal volume of reservoir solution: 18°C or 4°C, pH 5.6 , 19% isopropanol, 19% polyethylene glycol 4000, 5% glycerol, or 17% polyethylene glycol 4000, 5% glycerol | Leishmania tarentolae |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.4.2.8 | 0.0028 | - |
guanine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae | |
2.4.2.8 | 0.0044 | - |
hypoxanthine | recombinant enzyme, with 5-phospho-alpha-D-ribose 1-diphosphate | Leishmania tarentolae | |
2.4.2.8 | 0.127 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with guanine | Leishmania tarentolae | |
2.4.2.8 | 0.138 | - |
5-phosphoribosyl 1-diphosphate | recombinant enzyme, with hypoxanthine | Leishmania tarentolae |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | 23000 | - |
2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
2.4.2.8 | 50000 | - |
recombinant enzyme, gel filtration | Leishmania tarentolae |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | additional information | Leishmania tarentolae | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | ? | - |
? | |
2.4.2.8 | additional information | Leishmania tarentolae | enzyme is essential for salvaging exogenous purine bases | ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.4.2.8 | Leishmania tarentolae | Q9NJI5 | gene hgprt | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.4.2.8 | recombinant from Escherichia coli | Leishmania tarentolae |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.4.2.8 | guanine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | GMP + diphosphate | - |
? | |
2.4.2.8 | hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate | - |
Leishmania tarentolae | IMP + diphosphate | - |
? | |
2.4.2.8 | additional information | salvage incorporation of exogenous purine nucleotides, no de novo synthesis | Leishmania tarentolae | ? | - |
? | |
2.4.2.8 | additional information | enzyme is essential for salvaging exogenous purine bases | Leishmania tarentolae | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.4.2.8 | dimer | 2 * 23000, recombinant enzyme, SDS-PAGE | Leishmania tarentolae |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.4.2.8 | additional information | - |
recombinant enzyme, pI: 8.2 | Leishmania tarentolae |