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Literature summary extracted from

  • Heroux, A.; White, E.L.; Ross, L.J.; Kuzin, A.P.; Borhani, D.W.
    Substrate deformation in a hypoxanthine-guanine phosphoribosyltransferase ternary complex: the structural basis for catalysis (2000), Structure Fold. Des., 8, 1309-1318.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.8 medicine potential target for antiparasitic chemotherapy Toxoplasma gondii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8
-
 Toxoplasma gondii

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 the recombinant enzyme is complexed with Mg2+, 5-phosphoribosyl 1-diphosphate and inactive substrate analogue 9-deazaguanine, hanging drop method, enzyme complex, 20 mg/ml, is precipitated by 0.1 M Tris-HCl, pH 8.0, 30% polyethylene glycol 4000, 0.2 M Li2SO4, 0.5% beta-octylglucoside at 4°C, X-ray diffraction structure analysis Toxoplasma gondii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+
-
 Toxoplasma gondii

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Toxoplasma gondii
-
 IMP + diphosphate
-
 ?
2.4.2.8 additional information Toxoplasma gondii salvage incorporation of exogenous purine nucleotides, no de novo synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Toxoplasma gondii
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant enzyme Toxoplasma gondii

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate catalytic mechanism Toxoplasma gondii
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate active site structure Toxoplasma gondii
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii GMP + diphosphate
-
 r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Toxoplasma gondii IMP + diphosphate
-
 r
2.4.2.8 additional information 9-deazaguanine is no substrate Toxoplasma gondii ?
-
 ?
2.4.2.8 additional information salvage incorporation of exogenous purine nucleotides, no de novo synthesis Toxoplasma gondii ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 More structure determination and analysis Toxoplasma gondii