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Literature summary extracted from

  • Sujay Subbayya, I.N.; Sukumaran, S.; Shivashankar, K.; Balaram, H.
    Unusual substrate specificity of a chimeric hypoxanthine-guanine phosphoribosyltransferase containing segments from the Plasmodium falciparum and human enzymes (2000), Biochem. Biophys. Res. Commun., 272, 596-602.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study Homo sapiens
2.4.2.8 expression of wild-type and chimeric enzymes in enzyme-deficient Escherichia coli strain, complementation study, overexpression of chimeric mutant DS1 Plasmodium falciparum
2.4.2.8 overexpression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 additional information construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities Homo sapiens
2.4.2.8 additional information construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes, altered substrate specificities Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.001
-
 hypoxanthine wild-type enzyme Homo sapiens
2.4.2.8 0.0011
-
 guanine recombinant chimeric mutant DS1 Homo sapiens
2.4.2.8 0.0011
-
 guanine recombinant chimeric mutant DS1 Plasmodium falciparum
2.4.2.8 0.0014
-
 hypoxanthine recombinant chimeric mutant DS1 Homo sapiens
2.4.2.8 0.0014
-
 hypoxanthine recombinant chimeric mutant DS1 Plasmodium falciparum
2.4.2.8 0.005
-
 guanine wild-type enzyme Homo sapiens
2.4.2.8 0.3
-
 xanthine above, purified recombinant chimeric enzyme DS1 Homo sapiens
2.4.2.8 0.3
-
 xanthine above, purified recombinant chimeric enzyme DS1 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+
-
 Homo sapiens
2.4.2.8 Mg2+
-
 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens
-
 construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes
-
2.4.2.8 Plasmodium falciparum
-
 construction of 4 chimeric enzymes with segments of human and Plasmodium falciparum enzymes
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 0.032
-
 purified recombinant chimeric enzyme DS1, substrate guanine Homo sapiens
2.4.2.8 0.032
-
 purified recombinant chimeric enzyme DS1, substrate guanine Plasmodium falciparum
2.4.2.8 0.147
-
 purified recombinant chimeric enzyme DS1, substrate xanthine Homo sapiens
2.4.2.8 0.147
-
 purified recombinant chimeric enzyme DS1, substrate xanthine Plasmodium falciparum
2.4.2.8 0.226
-
 purified recombinant chimeric enzyme DS1, substrate hypoxanthine Homo sapiens
2.4.2.8 0.226
-
 purified recombinant chimeric enzyme DS1, substrate hypoxanthine Plasmodium falciparum
2.4.2.8 11.9
-
 purified recombinant enzyme, substrate hypoxanthine Homo sapiens
2.4.2.8 25.8
-
 purified recombinant enzyme, substrate guanine Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate no activity Homo sapiens XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate recombinant chimeric mutant DS1 Homo sapiens XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate recombinant chimeric mutant DS1 Plasmodium falciparum XMP + diphosphate
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 0.013
-
 guanine purified recombinant chimeric enzyme DS1 Homo sapiens
2.4.2.8 0.013
-
 guanine purified recombinant chimeric enzyme DS1 Plasmodium falciparum
2.4.2.8 0.062
-
 xanthine purified recombinant chimeric enzyme DS1 Homo sapiens
2.4.2.8 0.062
-
 xanthine purified recombinant chimeric enzyme DS1 Plasmodium falciparum
2.4.2.8 0.073
-
 hypoxanthine purified recombinant chimeric enzyme DS1 Homo sapiens
2.4.2.8 0.073
-
 hypoxanthine purified recombinant chimeric enzyme DS1 Plasmodium falciparum
2.4.2.8 5.7
-
 hypoxanthine wild-type enzyme Homo sapiens
2.4.2.8 10.5
-
 guanine wild-type enzyme Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 7.4
-
 assay at Homo sapiens
2.4.2.8 7.4
-
 assay at Plasmodium falciparum