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Literature summary extracted from

  • Keough, D.T.; Ng, A.L.; Winzor, D.J.; Emmerson, B.T.; de Jersey, J.
    Purification and characterization of Plasmodium falciparum hypoxanthine-guanine-xanthine phosphoribosyltransferase and comparison with the human enzyme (1999), Mol. Biochem. Parasitol., 98, 29-41.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.8 medicine potential target for antiparasitic chemotherapy Homo sapiens
2.4.2.8 medicine potential target for antiparasitic chemotherapy Plasmodium falciparum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 overexpression in an enzyme-deficient Escherichia coli strain Plasmodium falciparum
2.4.2.8 overexpression in Escherichia coli Homo sapiens

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 C105A prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage Homo sapiens
2.4.2.8 C205A prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage Homo sapiens
2.4.2.8 C22A prepared via splicing by overlap extension, reduced oxidation ofthe enzyme during storage Homo sapiens

General Stability

EC Number General Stability Organism
2.4.2.8 DTT and hypoxanthine stabilize Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 Hg2+ complete inhibition at 3 mM after 3 min at 0°C Homo sapiens
2.4.2.8 Hg2+ complete inhibition at 3 mM after 3 min at 0°C Plasmodium falciparum
2.4.2.8 KCl no inhibition Homo sapiens
2.4.2.8 KCl inactivation Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0009
-
 hypoxanthine recombinant enzyme, with other purine Plasmodium falciparum
2.4.2.8 0.0014
-
 guanine recombinant enzyme, with other purine Plasmodium falciparum
2.4.2.8 0.0019
-
 guanine recombinant enzyme, with other purine Homo sapiens
2.4.2.8 0.0031
-
 hypoxanthine recombinant enzyme, with other purine Homo sapiens
2.4.2.8 0.0117
-
 allopurinol recombinant enzyme Plasmodium falciparum
2.4.2.8 0.06
-
 hypoxanthine recombinant enzyme, with 5-phosphoribosyl 1-diphosphate Plasmodium falciparum
2.4.2.8 0.126
-
 hypoxanthine recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 Homo sapiens
2.4.2.8 0.134
-
 guanine recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 Homo sapiens
2.4.2.8 0.135
-
 allopurinol recombinant enzyme Homo sapiens
2.4.2.8 0.17
-
 xanthine recombinant enzyme, with 5-phosphoribosyl 1-diphosphate, pH 8.5 Plasmodium falciparum
2.4.2.8 0.362
-
 guanine recombinant enzyme, with 5-phosphoribosyl 1-diphosphate Plasmodium falciparum
2.4.2.8 0.42
-
 xanthine recombinant enzyme, pH 8.5 Plasmodium falciparum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+
-
 Homo sapiens
2.4.2.8 Mg2+
-
 Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Homo sapiens
-
-
-
2.4.2.8 Plasmodium falciparum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant from Escherichia coli Homo sapiens
2.4.2.8 recombinant from Escherichia coli , large scale Plasmodium falciparum

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 0.27
-
 purified recombinant enzyme, substrate allopurinol, pH 8.0 Homo sapiens
2.4.2.8 1.4
-
 purified recombinant enzyme, substrate hypoxanthine, pH 8.0 Plasmodium falciparum
2.4.2.8 3
-
 purified recombinant enzyme, substrates allopurinol and xanthine, pH 8.0 Plasmodium falciparum
2.4.2.8 5.4
-
 purified recombinant enzyme, substrate guanine, pH 8.0 Plasmodium falciparum
2.4.2.8 27
-
 purified recombinant enzyme, substrate hypoxanthine, pH 8.0 Homo sapiens
2.4.2.8 46
-
 purified recombinant enzyme, substrate guanine, pH 8.0 Homo sapiens

Storage Stability

EC Number Storage Stability Organism
2.4.2.8 0°C, 10 mM phosphate, pH 7.1, 1 mM DTT, 10 mM Mg2+, 2 h, no loss of activity Homo sapiens
2.4.2.8 4°C, freshly purified enzyme, 10 mM phosphate, pH 6.8, 10 mM DTT, 1 mM 5-phosphoribosyl 1-diphosphate, rapid loss of 90% activity within 48 h Plasmodium falciparum
2.4.2.8 5°C, purified enzyme, 10 mM phosphate, pH 6.8, 1 mM DTT, 0.2 mM 5-phosphoribosyl 1-diphosphate, 0.06 hypoxanthine, loss of 7% activity after 4-8 weeks Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum allopurinol ribonucleoside 5'-monophosphate + diphosphate
-
 ?
2.4.2.8 allopurinol + 5-phospho-alpha-D-ribose 1-diphosphate low activity Homo sapiens allopurinol ribonucleoside 5'-monophosphate + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum IMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens XMP + diphosphate
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Plasmodium falciparum XMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 ? x * 24352-24353, recombinant mutant enzymes, mass spectroscopy and DNA sequence determination Homo sapiens
2.4.2.8 dimer
-
 Homo sapiens
2.4.2.8 dimer 2 * 26229-26232, in presence of KCl, SDS-PAGE, mass spectroscopy and DNA sequence determination Plasmodium falciparum
2.4.2.8 tetramer
-
 Homo sapiens
2.4.2.8 tetramer 4 * 26229-26232, in absence of KCl, SDS-PAGE, mass spectroscopy and DNA sequence determination Plasmodium falciparum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 25
-
 assay at Plasmodium falciparum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 8.5
-
 assay at Plasmodium falciparum