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Literature summary extracted from

  • Shih, S.; Hwang, H.Y.; Carter, D.; Stenberg, P.; Ullman, B.
    Localization and targeting of the Leishmania donovani hypoxanthine-guanine phosphoribosyltransferase to the glycosome (1998), J. Biol. Chem., 273, 1534-1541.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 functional expression of wild-type enzyme and mutants in an enzyme-deficient Escherichia coli strain Leishmania donovani

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 additional information construction of mutant lacking the Ser-Lys-Val C-terminal targeting signal, mutant enzyme is located throughout the parasite, including subcellular organelles such as nucleus and flagellum Leishmania donovani

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.0048
-
 hypoxanthine mutant enzyme Leishmania donovani
2.4.2.8 0.0064
-
 hypoxanthine wild-type enzyme Leishmania donovani
2.4.2.8 0.01
-
 guanine wild-type enzyme Leishmania donovani
2.4.2.8 0.012
-
 guanine mutant enzyme Leishmania donovani

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.2.8 glycosome a fuel-metabolizing microbody unique to this parasite, exclusively Leishmania donovani 20015
-
2.4.2.8 additional information the mutant enzyme, lacking the targeting signal, is located throughout the parasite, including subcellular organelles such as nucleus and flagellum Leishmania donovani
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Leishmania donovani
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Leishmania donovani
-
 IMP + diphosphate
-
 ?
2.4.2.8 additional information Leishmania donovani salvage incorporation of exogenous purine nucleotides, no de novo synthesis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Leishmania donovani
-
 parasites isolated from rabbits
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant wild-type and mutant from Escherichia coli Leishmania donovani

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 promastigote
-
 Leishmania donovani
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani GMP + diphosphate
-
 r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Leishmania donovani IMP + diphosphate
-
 r
2.4.2.8 additional information salvage incorporation of exogenous purine nucleotides, no de novo synthesis Leishmania donovani ?
-
 ?

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 5.7
-
 hypoxanthine wild-type enzyme Leishmania donovani
2.4.2.8 6.2
-
 hypoxanthine mutant enzyme Leishmania donovani
2.4.2.8 12.1
-
 guanine wild-type enzyme Leishmania donovani
2.4.2.8 15.8
-
 guanine mutant enzyme Leishmania donovani