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Literature summary extracted from

  • Munagala, N.R.; Chin, M.S.; Wang, C.C.
    Steady-state kinetics of the hypoxanthine-guanine-xanthine phosphoribosyltransferase from Tritrichomonas foetus: the role of threonine-47 (1998), Biochemistry, 37, 4045-4051.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.4.2.8 medicine target for anti-trichomonial therapy Tritrichomonas suis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.2.8 functional expression of wild-type and mutant T47K in an enzyme-deficient Escherichia coli strain Tritrichomonas suis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.4.2.8 analysis of crystal structure Tritrichomonas suis

Protein Variants

EC Number Protein Variants Comment Organism
2.4.2.8 T47K site-directed mutagenesis, exchange of diphosphate binding site residue, 4-10fold decreased Km for diphosphate compared to the wild-type Tritrichomonas suis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.2.8 5-phospho-alpha-D-ribose 1-diphosphate product inhibition Tritrichomonas suis
2.4.2.8 diphosphate product inhibition Tritrichomonas suis
2.4.2.8 GMP product inhibition Tritrichomonas suis
2.4.2.8 IMP product inhibition Tritrichomonas suis
2.4.2.8 XMP product inhibition Tritrichomonas suis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 additional information
-
 additional information kinetics Tritrichomonas suis
2.4.2.8 additional information
-
 additional information wild-type and mutant T47K Tritrichomonas suis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate Tritrichomonas suis
-
 GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Tritrichomonas suis
-
 IMP + diphosphate
-
 ?
2.4.2.8 additional information Tritrichomonas suis salvage incorporation of exogenous purine nucleotides, no de novo synthesis ?
-
 ?
2.4.2.8 additional information Tritrichomonas suis enzyme is essential for salvaging exogenous purine bases ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Tritrichomonas suis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8 recombinant wild-type enzyme and mutant/s from Escherichia coli Tritrichomonas suis

Reaction

EC Number Reaction Comment Organism Reaction ID
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate ordered bi-bi mechanism Tritrichomonas suis
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate Thr47 binds diphosphate Tritrichomonas suis
a
2.4.2.8 IMP + diphosphate = hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate kinetic model Tritrichomonas suis
a

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 additional information
-
 kinetics in forward and reverse reaction Tritrichomonas suis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Tritrichomonas suis GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Tritrichomonas suis GMP + diphosphate
-
 r
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Tritrichomonas suis IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate most effective substrate Tritrichomonas suis IMP + diphosphate diphosphate is bound by Thr47 r
2.4.2.8 additional information salvage incorporation of exogenous purine nucleotides, no de novo synthesis Tritrichomonas suis ?
-
 ?
2.4.2.8 additional information enzyme is essential for salvaging exogenous purine bases Tritrichomonas suis ?
-
 ?
2.4.2.8 xanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Tritrichomonas suis XMP + diphosphate
-
 r

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.4.2.8 6.71
-
 hypoxanthine mutant T47K Tritrichomonas suis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.4.2.8 additional information
-
 additional information kinetics of product inhibition in forward reaction Tritrichomonas suis