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Literature summary extracted from

  • Olsen, A.S.; Milman, G.
    Hypoxanthine phosphoribosyltransferase from Chinese hamster brain and human erythrocytes (1978), Methods Enzymol., 51, 543-549.
    View publication on PubMed

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.2.8 0.00052
-
 hypoxanthine
-
 Cricetulus griseus
2.4.2.8 0.0011
-
 guanine
-
 Cricetulus griseus
2.4.2.8 0.0011
-
 guanine
-
 Homo sapiens
2.4.2.8 0.0053
-
 5-phospho-alpha-D-ribose 1-diphosphate
-
 Cricetulus griseus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.2.8 Mg2+ required Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.2.8 25000
-
 3 * 25000, SDS-PAGE Cricetulus griseus
2.4.2.8 26000
-
 3 * 26000, SDS-PAGE Homo sapiens
2.4.2.8 78000 85000 gel filtration Cricetulus griseus
2.4.2.8 80000 85000 gel filtration, acrylamide gel electrophoresis Cricetulus griseus
2.4.2.8 81000 83000 sedimentation equilibrium centrifugation Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
2.4.2.8 Cricetulus griseus
-
 at least 3 charge variant isoforms
-
2.4.2.8 Homo sapiens
-
 3 charge variant forms
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.2.8
-
 Cricetulus griseus
2.4.2.8 from erythrocytes and from brain Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.2.8 brain
-
 Cricetulus griseus
-
2.4.2.8 erythrocyte
-
 Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.2.8 9
-
 purified enzyme, brain Cricetulus griseus
2.4.2.8 17.5
-
 purified enzyme, erythrocytes Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Cricetulus griseus GMP + diphosphate
-
 ?
2.4.2.8 guanine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens GMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Cricetulus griseus IMP + diphosphate
-
 ?
2.4.2.8 hypoxanthine + 5-phospho-alpha-D-ribose 1-diphosphate
-
 Homo sapiens IMP + diphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
2.4.2.8 trimer 3 * 26000, SDS-PAGE Homo sapiens
2.4.2.8 trimer 3 * 25000, SDS-PAGE Cricetulus griseus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.2.8 37
-
 assay at Cricetulus griseus
2.4.2.8 37
-
 assay at Homo sapiens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.2.8 85
-
 if first incubated in 1 mM 5-phospho-alpha-D-ribose 1-diphosphate, remarkably stable Cricetulus griseus
2.4.2.8 85
-
 if first incubated in 1 mM 5-phospho-alpha-D-ribose 1-diphosphate, remarkably stable Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.2.8 additional information
-
 3 charge variant forms with pIs of 5.6, 5.7 and 5.9 Homo sapiens
2.4.2.8 additional information
-
 3 charge variant forms with pIs of 6.2, 6.4 and 6.6 Cricetulus griseus
2.4.2.8 7.8
-
 assay at Homo sapiens
2.4.2.8 10
-
-
 Cricetulus griseus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.2.8 5.5 11
-
 Cricetulus griseus