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Literature summary extracted from

  • Holmes, E.H.
    Characterization and membrane organization of beta 1-3- and beta 1-4-galactosyltransferases from human colonic adenocarcinoma cell lines Colo 205 and SW403: basis for preferential synthesis of type 1 chain lacto-series carbohydrate structures (1989), Arch. Biochem. Biophys., 270, 630-646.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.4.1.179 Bovine liver non-specific transfer proteins activation Homo sapiens
2.4.1.179 CHAPSO 5 to 7fold less effective compared to Triton CF-54 Homo sapiens
2.4.1.179 deoxycholate 5 to 7fold less effective compared to Triton CF-54 Homo sapiens
2.4.1.179 additional information little or no activation by phosphatidylcholine, phosphatidylglycerol, phosphatidylserine, phosphatidylinositol or cardiolipin, Brij-58, Empigen BB or detergent G-3634-A Homo sapiens
2.4.1.179 phosphatidylethanolamine activation, less efficient than Triton CF-54 Homo sapiens
2.4.1.179 taurodeoxycholate 5 to 7fold less effective compared to Triton CF-54 Homo sapiens
2.4.1.179 Triton CF-54 activation, optimal at 0.1% Homo sapiens
2.4.1.179 Triton X-100 activation, can replace Triton CF-54 with 75% less efficiency Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.179 0.013
-
 lactotriaosylceramide
-
 Homo sapiens
2.4.1.179 0.048
-
 UDP-galactose
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.4.1.179 endoplasmic reticulum
-
 Homo sapiens 5783
-
2.4.1.179 Golgi apparatus
-
 Homo sapiens 5794
-
2.4.1.179 membrane
-
 Homo sapiens 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.179 Ca2+ activation, can replace Mn2+ with 26% efficiency Homo sapiens
2.4.1.179 Co2+ activation, can replace Mn2+ with 51% efficiency Homo sapiens
2.4.1.179 Cu2+ activation, can replace Mn2+ with 15% efficiency Homo sapiens
2.4.1.179 Mg2+ activation, can replace Mn2+ with 13% efficiency Homo sapiens
2.4.1.179 Mn2+ requirement, 25 mM Homo sapiens
2.4.1.179 additional information no activation by Zn2+ or Cd2+ Homo sapiens
2.4.1.179 Ni2+ activation, can replace Mn2+ with 16% efficiency Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid Homo sapiens associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.179 Homo sapiens
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.4.1.179 from Colo 205 cell line Homo sapiens
2.4.1.179 partial Homo sapiens

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.179 cell suspension culture
-
 Homo sapiens
-
2.4.1.179 Colo-205 cell colonic adenocarcino cell line Homo sapiens
-
2.4.1.179 colonic adenocarcinoma cell
-
 Homo sapiens
-
2.4.1.179 SW-403 cell colonic adenocarcino cell line Homo sapiens
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.179 0.00077
-
-
 Homo sapiens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-glycolipid associated with biosynthesis of type 1 lactoseries core chain carbohydrate structure Homo sapiens UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-glycolipid
-
 ?
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R substrate specificity Homo sapiens UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
 ?
2.4.1.179 UDP-galactose + lactotriaosylceramide best substrate Homo sapiens UDP + lactotetraosylceramide
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.179 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.179 7
-
 Hepes buffer Homo sapiens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.179 5.8 8.2 about half-maximal activity at pH 5.8 and pH 8.2, cacodylate or Tris-HCl buffer Homo sapiens