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Literature summary extracted from

  • Messer, M.; Nicholas, K.R.
    Biosynthesis of marsupial milk oligosaccharides: characterization and developmental changes of two galactosyltransferases in lactating mammary glands of the tammar wallaby, Macropus eugenii (1991), Biochim. Biophys. Acta, 1077, 79-85.
    View publication on PubMed

General Stability

EC Number General Stability Organism
2.4.1.179 Mn2+ enhances thermal stability Notamacropus eugenii

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.4.1.179 EDTA 97% inhibition at 30 mM Notamacropus eugenii
2.4.1.179 additional information bovine or gammar alpha-lactalbumin Notamacropus eugenii

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.179 46
-
 lactose
-
 Notamacropus eugenii

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.179 Mn2+ absolute requirement Notamacropus eugenii

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.179 no activity in Mus musculus
-
 lactating mammary gland
-
2.4.1.179 Notamacropus eugenii
-
 female tammar wallaby
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.4.1.179 mammary gland
-
 Notamacropus eugenii
-
2.4.1.179 additional information dramatic changes during lactation Notamacropus eugenii
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.4.1.179 0.02 0.032 from day 90 to day 225 post partum Notamacropus eugenii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.179 additional information glucose is a very poor acceptor substrate Notamacropus eugenii ?
-
 ?
2.4.1.179 UDP-galactose + beta-D-galactose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-beta-D-galactose
-
 ?
2.4.1.179 UDP-galactose + beta-D-galactosyl-1,6-N-acetylglucosylamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-beta-D-galactosyl-1,6-N-acetylglucosylamine
-
 ?
2.4.1.179 UDP-galactose + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucose best substrate Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucose i.e. lactose Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucose
-
 ?
2.4.1.179 UDP-galactose + D-galactosyl-1,4-beta-D-glucosyl-R
-
 Notamacropus eugenii UDP + D-galactosyl-1,3-beta-D-galactosyl-1,4-beta-D-glucosyl-R
-
 ?
2.4.1.179 UDP-galactose + lacto-N-tetraose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-lacto-N-tetraose
-
 ?
2.4.1.179 UDP-galactose + lactulose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-lactulose
-
 ?
2.4.1.179 UDP-galactose + melibiose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-melibiose
-
 ?
2.4.1.179 UDP-galactose + methyl-alpha-galactoside
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-methyl-alpha-galactoside
-
 ?
2.4.1.179 UDP-galactose + methyl-beta-galactoside
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-methyl-beta-galactoside
-
 ?
2.4.1.179 UDP-galactose + N-acetylgalactosamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetylgalactosamine
-
 ?
2.4.1.179 UDP-galactose + N-acetyllactosamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetyllactosamine
-
 ?
2.4.1.179 UDP-galactose + p-nitrophenyl-alpha-N-acetylgalactosylamine
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-N-acetyl-alpha-galactosylaminyl-4-nitrophenyl
-
 ?
2.4.1.179 UDP-galactose + stachyose
-
 Notamacropus eugenii UDP + beta-D-galactosyl-1,3-stachyose
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.4.1.179 UDP-galactose:lactose beta1,3-galactosyltransferase
-
 Notamacropus eugenii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.179 37
-
-
 Notamacropus eugenii

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.4.1.179 36
-
 t1/2: 20 min Notamacropus eugenii
2.4.1.179 39
-
 30 min, complete inactivation, in the presence of Mn2+ 11% loss of activity within 60 min Notamacropus eugenii

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.179 6.5
-
-
 Notamacropus eugenii

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.179 5 8 about 75% of maximal activity at pH 5 and about half-maximal activity at pH 8.0 Notamacropus eugenii